Multifunctional hydrogel based on dopamine-modified hyaluronic acid, gelatin and silver nanoparticles for promoting abdominal wall defect repair

Abdominal wall defects are often accompanied by severe infections and complications, creating a significant challenge for clinicians. There is an urgent need to develop a novel wound dressing that can effectively prevent intra-abdominal infection and promote the healing of defective abdominal walls. Based on a hydrogel dressing containing hyaluronic acid (HA) and gelatin (GT), herein we integrated dopamine with a catechol structure to enhance its antioxidant and adherent properties. HA is oxidized to form an aldehyde group, and subsequently grafted with dopamine. The dopamine-modified HA undergoes amidation reaction with GT at different con-centrations. In addition, silver nanoparticles (AgNPs) were introduced to the hydrogel to enhance the antibac-terial properties. The in vitro studies on GT/DA-HA demonstrated excellent physical and chemical properties, biodegradability, and biocompatibility. In a rat full-thickness skin defect model and a full-thickness abdominal wall defect model, the GT/DA-HA hydrogel could accelerate the healing process by improving wet adhesion, reducing wound inflammation, and promoting angiogenesis and formation of granulation tissues. The multi-functional hydrogel developed in this study shows great potential for treating full-thickness abdominal wall defects.

腹壁缺损常伴有严重感染和并发症，这给临床医生带来了重大挑战。迫切需要开发一种新型伤口敷料，能够有效预防腹腔内感染并促进腹壁缺损的愈合。基于一种含有透明质酸（HA）和明胶（GT）的水凝胶敷料，在此我们将具有儿茶酚结构的多巴胺整合进去以增强其抗氧化和黏附性能。透明质酸被氧化形成醛基，随后与多巴胺接枝。经多巴胺修饰的透明质酸与不同浓度的明胶发生酰胺化反应。此外，将银纳米粒子（AgNPs）引入水凝胶以增强抗菌性能。对GT/DA - HA的体外研究表明其具有优异的物理和化学性质、生物降解性以及生物相容性。在大鼠全层皮肤缺损模型和全层腹壁缺损模型中，GT/DA - HA水凝胶可通过提高湿黏附性、减轻伤口炎症以及促进血管生成和肉芽组织形成来加速愈合过程。本研究中开发的多功能水凝胶在治疗全层腹壁缺损方面显示出巨大潜力。