TAZ is downregulated by dexamethasone during the differentiation of 3T3-L1 preadipocytes

TAZ (transcriptional co-activator with PDZ binding motif) is a transcriptional modulator of mesenchymal stem cell differentiation. We have found that TAZ was expressed in postconfluent 3T3-L1 preadipocytes and downregulated during differentiation. Downregulation of TAZ was specifically mediated by dexamethasone (DEX), one component of induction cocktails routinely used in adipocyte differentiation. DEX repressed the transcription of TAZ by direct binding of the glucocorticoid receptor (GR) to the GR binding element in its promoter. More importantly, overexpression of TAZ inhibited adipogenesis and promoted the trans-differentiation of preadipocytes into osteocytes. This establishes a new functional interaction between DEX and TAZ that contributes to the mechanism of adipogenesis. (C) 2012 Elsevier Inc. All rights reserved.

TAZ（具有PDZ结合基序的转录共激活因子）是间充质干细胞分化的转录调节因子。我们发现TAZ在汇合后的3T3 - L1前脂肪细胞中表达，并在分化过程中下调。TAZ的下调是由地塞米松（DEX）特异性介导的，地塞米松是脂肪细胞分化常规诱导混合剂中的一种成分。地塞米松通过糖皮质激素受体（GR）与其启动子中的GR结合元件直接结合来抑制TAZ的转录。更重要的是，TAZ的过表达抑制脂肪生成，并促进前脂肪细胞向骨细胞的转分化。这确立了地塞米松和TAZ之间一种新的功能相互作用，这种相互作用有助于脂肪生成的机制。（C）2012爱思唯尔公司。保留所有权利。