基于激光散斑对比成像技术的化痰通络法对脑梗死大鼠脑血流的影响

Objective: To observe the effect of the phlegm-resolving and collateral-unblocking method on cerebral blood flow in rats with acute cerebral infarction, and to provide some experimental basis for revealing its mechanism of action in treating cerebral infarction. Methods: Twenty-seven SD rats were randomly divided into a sham operation group, a model group and a Chinese medicine group. The MCAO model was prepared by the suture-occluded method, and each group was given intragastric administration. Images were collected by a laser speckle contrast analysis (LSCA) system at corresponding time points, and the relative cerebral blood flow velocity was calculated. Results: LSCA showed that the cerebral blood flow velocity of rats decreased significantly 1 day after modeling; in the Chinese medicine group, a significant increase in blood flow was observed at 3 days, and the blood flow velocity was significantly accelerated at 7 days; the data showed that the cerebral blood flow in the Chinese medicine group also decreased significantly at 1 day, but increased significantly at 3 days and was significantly higher than that in the model group; at 7 days, the cerebral blood flow velocity in the Chinese medicine group continued to increase and was significantly higher than that in the model group and the sham operation group. Conclusion: The compound recipe of the phlegm-resolving and collateral-unblocking method can significantly increase the cerebral blood flow velocity in rats with cerebral infarction, which may be helpful for improving the symptoms of neurological function deficits in model rats.

目的:观察化痰通络法对急性脑梗死大鼠脑血流的影响,为揭示其治疗脑梗死的作用机制提供部分实验依据。方法:将27只SD大鼠随机分为假手术组、模型组和中药组,采用线栓法制备MCAO模型,各组采用灌胃给药。在相应时间点采用激光散斑对比成像(LSCA)系统采集图像,计算相对脑血流速度。结果: LSCA显示,造模1 d后大鼠脑血流速度下降明显;中药组在3 d时即见明显血流增加,7 d时血流速度明显加快;数据显示,中药组脑血流在1 d亦明显降低,但在3 d时明显增高,且明显高于模型组; 7 d时中药组脑血流速度继续升高,明显高于模型组和假手术组。结论:化痰通络法复方可明显提升脑梗死大鼠脑血流速度,这可能有助于改善模型大鼠神经功能缺损症状。