Identification and characterization of human non-coding RNAs with tissue-specific expression

We have examined the expression profile of selected non-coding RNAs (ncRNAs) in 11 human tissues. Among 5489 full-length cDNA clones annotated as non-protein-coding transcripts in the H-Invitational database, we chose 150 clones for further analysis based on their gene structure and EST information. Expression profiling using quantitative RT-PCR and Northern blot hybridization revealed that the majority of the selected ncRNAs exhibited tissue specificity: 67% are predominantly expressed in a restricted subset of tissues. The absolute quantification of representative ncRNAs revealed that the majority of ncRNAs are expressed as low abundance transcripts. A comparative genomic analysis revealed that only 27% of the selected ncRNAs have mouse counterparts. Since the expression patterns of the human ncRNAs having no mouse counterparts remain to be similar to those of the mouse ncRNAs, the expression patterns of the selected ncRNAs may be conserved between human and mouse. (c) 2007 Elsevier Inc. All rights reserved.

我们检测了11种人体组织中选定的非编码RNA（ncRNAs）的表达谱。在H - 国际协作组数据库中被标注为非蛋白质编码转录本的5489个全长cDNA克隆中，我们根据基因结构和EST信息选择了150个克隆进行进一步分析。利用定量RT - PCR和Northern杂交进行的表达谱分析显示，大多数选定的非编码RNA表现出组织特异性：67%主要在有限的组织子集中表达。对代表性非编码RNA的绝对定量分析表明，大多数非编码RNA作为低丰度转录本表达。一项比较基因组分析显示，只有27%的选定非编码RNA在小鼠中有对应物。由于没有小鼠对应物的人类非编码RNA的表达模式与小鼠非编码RNA的表达模式仍然相似，所以选定的非编码RNA的表达模式在人类和小鼠之间可能是保守的。（c）2007爱思唯尔公司。保留所有权利。