A Quinoline-Appended Cyclodextrin Derivative as a Highly Selective Receptor and Colorimetric Probe for Nucleotides

The design and development of specific recognition and sensing systems for biologically important anionic species has received growing attention in recent years, as they play significant roles in biology, pharmacy, and environmental sciences. Herein, a new supramolecular sensing probe L1 was developed for highly selective differentiation of nucleotides. L1 displayed extremely marked absorption and emission differentiation upon binding with nucleotide homologs of AMP, ADP, and ATP, due to the divergent spatial orientations of guests upon binding, which allowed for a naked-eye colorimetric differentiation for nucleotides. A differentiating mechanism was unambiguously rationalized by using various spectroscopic studies and theoretical calculations. Furthermore, we successfully demonstrated that L1 can be applied to the real-time monitoring of the enzyme-catalyzed phosphorylation/dephosphorylation processes and thus demonstrated an unprecedented visualizable strategy for selectively differentiating the structurally similar nucleotides and real-time monitoring of biological processes via fluorescent and colorimetric changes.

近年来，针对具有重要生物学意义的阴离子物种的特异性识别和传感系统的设计与开发受到了越来越多的关注，因为它们在生物学、药学和环境科学中发挥着重要作用。在此，一种新型超分子传感探针L1被开发用于对核苷酸进行高选择性区分。L1在与AMP、ADP和ATP的核苷酸同系物结合时，由于客体在结合时的空间取向不同，表现出极其显著的吸收和发射差异，这使得能够通过肉眼比色区分核苷酸。通过各种光谱研究和理论计算，明确地阐明了一种区分机制。此外，我们成功地证明了L1可应用于酶催化的磷酸化/去磷酸化过程的实时监测，从而展示了一种前所未有的可视化策略，用于通过荧光和比色变化选择性地区分结构相似的核苷酸以及实时监测生物过程。