Oxygen-Sensing Paint-On Bandage: Calibration of a Novel Approach in Tissue Perfusion Assessment

Background: Knowledge of tissue oxygenation status is fundamental in the prevention of postoperative flap failure. Recently, the authors introduced a novel oxygen-sensing paint-on bandage that incorporated an oxygen-sensing porphyrin with a commercially available liquid bandage matrix. In this study, the authors extend validation of their oxygen-sensing bandage by comparing it to the use of near-infrared tissue oximetry in addition to Clark electrode measurements. Methods: The oxygen-sensing paint-on bandage was applied to the left hind limb in a rodent model. Simultaneously, a near-infrared imaging device and Clark electrode were attached to the right and left hind limbs, respectively. Tissue oxygenation was measured under normal, ischemic (aortic ligation), and reperfused conditions. Results: On average, the oxygen-sensing paint-on bandage measured a decrease in transdermal oxygenation from 85.2 mmHg to 64.1 mmHg upon aortic ligation. The oxygen-sensing dye restored at 81.2 mmHg after unclamping. Responses in both control groups demonstrated a similar trend. Physiologic changes from normal to ischemic and reperfused conditions were statistically significantly different in all three techniques (p < 0.001). Conclusions: The authors’ newly developed oxygen-sensing paint-on bandage exhibits a comparable trend in oxygenation recordings in a rat model similar to conventional oxygenation assessment techniques. This technique could potentially prove to be a valuable tool in the routine clinical management of flaps following free tissue transfer. Incorporating oxygen-sensing capabilities into a simple wound dressing material has the added benefit of providing both wound protection and constant wound oxygenation assessment.

背景：了解组织氧合状态对于预防术后皮瓣失败至关重要。近期，作者引入了一种新型的涂覆式氧传感绷带，它将一种氧传感卟啉与一种市售的液体绷带基质相结合。在本研究中，作者通过将其与近红外组织血氧测定法以及克拉克电极测量法进行比较，进一步验证了他们的氧传感绷带。 方法：在啮齿动物模型中，将氧传感涂覆式绷带应用于左后肢。同时，将近红外成像设备和克拉克电极分别连接到右后肢和左后肢。在正常、缺血（主动脉结扎）和再灌注条件下测量组织氧合情况。 结果：平均而言，氧传感涂覆式绷带测得在主动脉结扎时经皮氧合从85.2 mmHg下降到64.1 mmHg。松开夹子后，氧传感染料恢复到81.2 mmHg。两个对照组的反应呈现出相似的趋势。从正常到缺血和再灌注条件的生理变化在所有三种技术中均具有统计学显著差异（p < 0.001）。 结论：作者新开发的氧传感涂覆式绷带在大鼠模型中的氧合记录呈现出与传统氧合评估技术相似的趋势。该技术可能被证明是游离组织移植后皮瓣常规临床管理中的一种有价值的工具。将氧传感能力整合到一种简单的伤口敷料材料中具有额外的优势，即既能提供伤口保护，又能持续评估伤口氧合情况。