A dataset of dual calcium and voltage optical mapping in healthy and hypertrophied murine hearts.

Pathological hypertrophy underlies sudden cardiac death due to its high incidence of occurrence of ventricular arrhythmias. The alteration of transmural electrophysiological properties in hypertrophic cardiac murine tissue has never been explored previously. In this dataset, we have for the first time conducted high-throughput simultaneous optical imaging of transmembrane potential and calcium transients (CaT) throughout the entire hypertrophic murine hearts at high temporal and spatial resolution. Using ElectroMap, we have conducted multiple parameters analysis including action potential duration/calcium transient duration, conduction velocity, alternans and diastolic interval. Voltage-calcium latency was measured as time difference between action potential and CaT peak. The dataset therefore provides the first high spatial resolution transmural electrophysiological profiling of the murine heart, allowing interrogation of mechanisms driving ventricular arrhythmias associated with pathological hypertrophy. The dataset allows for further reuse and detailed analyses of geometrical, topological and functional analyses and reconstruction of 2-dimensional and 3-dimentional models. Machine-accessible metadata file describing the reported data: 10.6084/m9.figshare.16910542

病理性肥大是心脏性猝死的基础，因为其室性心律失常的发生率很高。此前从未探索过肥大心脏小鼠组织透壁电生理特性的改变。在本数据集中，我们首次以高时空分辨率对整个肥大小鼠心脏的跨膜电位和钙瞬变（CaT）进行了高通量同步光学成像。利用ElectroMap，我们进行了多个参数分析，包括动作电位时程/钙瞬变时程、传导速度、交替现象和舒张间期。电压 - 钙潜伏期以动作电位和CaT峰值之间的时间差来衡量。因此，该数据集首次提供了小鼠心脏的高空间分辨率透壁电生理图谱，有助于探究病理性肥大相关室性心律失常的驱动机制。该数据集允许进一步重复使用，并对几何、拓扑和功能进行详细分析以及二维和三维模型的重建。 描述报告数据的机器可读元数据文件：10.6084/m9.figshare.16910542