Preservation of Capillary-beds in Rat Lung Tissue Using Optimized Chemical Decellularization.

Promoting regeneration using scaffolds created by decellularizing native tissue is becoming a popular technique applied to a variety of tissues. We demonstrate a method to decellularize highly vascular tissue keeping the vascular structure intact down to the capillary scale. Using vascular corrosion casting (VCC), we created a method for quantitatively assessing the functionality of vascular extracellular matrix (ECM) following decellularization. Murine lung tissue was decellularized using a number of techniques, then characterized using standard histological methods, as well as our quantitative VCC (qVCC) technique. Using an optimized acellular method, we successfully decellularized lung tissue while leaving behind a patent vascular network based on qualitative and quantitative histological methods.

利用对天然组织进行脱细胞处理制成的支架来促进再生，正在成为一种应用于多种组织的流行技术。我们展示了一种对高度血管化组织进行脱细胞处理的方法，该方法能使血管结构在毛细血管级别仍保持完整。通过血管铸型（VCC）技术，我们创建了一种在脱细胞处理后对血管细胞外基质（ECM）功能进行定量评估的方法。我们使用多种技术对小鼠肺组织进行脱细胞处理，然后利用标准的组织学方法以及我们的定量血管铸型（qVCC）技术对其进行表征。通过一种优化的脱细胞方法，我们基于定性和定量组织学方法，成功地对肺组织进行了脱细胞处理，同时保留了一个通畅的血管网络。