Boronate affinity Metal-Organic frameworks for highly efficient cis-diol molecules in-situ enrichment and surface-assisted laser desorption/ionization mass spectrometric detection

Development of novel matrix for surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) is a hot-spot in research frontiers. Herein, we established novel SALDI-MS approach for the analysis of cis-diol small molecules by using boronate affinity metal-organic frameworks (BA-MOFs) as the sor-bent for extraction and later as matrix for SALDI. In the meantime, the prepared BA-MOFs exhibited several significant merits, such as excellent selectivity, tolerance to interference and fast binding equilibria. By integrating the advantages of MOFs and the molecular recognition of boronate affinity, a novel platform for simultaneous enrichment and MS detection of cis-diol small molecules was proposed for the first time. There are only two steps in the operation procedure, which greatly simplified the whole experimental workflow. Efficient analysis of cis-diol small molecules in real samples were also well demonstrated. This proposed novel protocol not only gave new insights into the application of functionalized MOFs, but also promoted the development of SALDI- MS. (C) 2019 Elsevier B.V. All rights reserved.

表面辅助激光解吸/电离质谱（SALDI - MS）新型基质的开发是研究前沿的热点。在此，我们建立了一种新的SALDI - MS方法，用于分析顺式二醇小分子，该方法使用硼酸亲和金属 - 有机框架（BA - MOFs）作为萃取吸附剂，随后作为SALDI的基质。同时，所制备的BA - MOFs具有若干显著优点，例如优异的选择性、抗干扰能力和快速的结合平衡。通过整合MOFs的优势以及硼酸亲和的分子识别能力，首次提出了一个用于顺式二醇小分子同时富集和质谱检测的新平台。操作过程仅有两步，极大地简化了整个实验流程。在实际样品中对顺式二醇小分子的高效分析也得到了很好的验证。所提出的新方案不仅为功能化MOFs的应用提供了新的见解，还促进了SALDI - MS的发展。（C）2019爱思唯尔有限公司。保留所有权利。