Protocol for remapping of place cells in disease mouse models.

Hippocampal place cells and entorhinal grid cells exhibit distinct spike patterns in different environments called “remapping,” and we have recently shown that remapping of place cells becomes disrupted in a mouse model of Alzheimer's disease. Here, we describe our protocol for investigating remapping of place cells and grid cells using a custom-made electrophysiology device, with detailed descriptions and problem-solving tips for the construction and implantation of the recording device. We also provide steps for behavioral training, recording, and data analysis. For complete details on the use and execution of this protocol, please refer to Build behavior apparatuses for remapping experiment and construct microdrives Implant drives, monitor animal recovery, and perform tetrode turning Perform behavior training followed by recording on fifth day Analyze data to identify place cells and evaluate degree of remapping Hippocampal place cells and entorhinal grid cells exhibit distinct spike patterns in different environments called “remapping,” and we have recently shown that remapping of place cells becomes disrupted in a mouse model of Alzheimer's disease. Here, we describe our protocol for investigating remapping of place cells and grid cells using a custom-made electrophysiology device, with detailed descriptions and problem-solving tips for the construction and implantation of the recording device. We also provide steps for behavioral training, recording, and data analysis.

海马位置细胞和内嗅皮层网格细胞在不同环境中呈现出不同的放电模式，称为“重映射”，我们最近发现，在阿尔茨海默病小鼠模型中，位置细胞的重映射受到了干扰。在此，我们描述了使用定制的电生理设备研究位置细胞和网格细胞重映射的实验方案，包括记录设备构建和植入的详细说明以及问题解决技巧。我们还提供了行为训练、记录和数据分析的步骤。 如需此方案使用和实施的完整细节，请参考： 为重映射实验构建行为装置并制作微驱动器 植入驱动器，监测动物恢复情况，并进行四极管旋转 进行行为训练，随后在第五天进行记录 分析数据以识别位置细胞并评估重映射程度 海马位置细胞和内嗅皮层网格细胞在不同环境中呈现出不同的放电模式，称为“重映射”，我们最近发现，在阿尔茨海默病小鼠模型中，位置细胞的重映射受到了干扰。在此，我们描述了使用定制的电生理设备研究位置细胞和网格细胞重映射的实验方案，包括记录设备构建和植入的详细说明以及问题解决技巧。我们还提供了行为训练、记录和数据分析的步骤。