Uniformly colorized beads for multiplex immunoassay

We introduced a fluidic droplet generation method for the generation of monodispersed polymer beads with structural color. Multicolorized polystyrene beads were derived by embedding pearl pigment. Methods for generating uniform color and controlling bead size and surface were developed to make the beads suitable for multiplex immunoassay. A multiplex assay of antigen-antibody interaction shows that the multicolored encoded bead is a stable and cost-effective microcarrier. Wide applications in genomic or proteomic studies, drug screening, clinical diagnostics, and combinatorial chemical fields are anticipated.

我们引入了一种流体液滴生成方法，用于生成具有结构色的单分散聚合物微珠。通过嵌入珠光颜料得到了多彩聚苯乙烯微珠。开发了产生均匀颜色以及控制微珠尺寸和表面的方法，以使微珠适用于多重免疫分析。抗原 - 抗体相互作用的多重分析表明，多彩编码微珠是一种稳定且具有成本效益的微载体。预计在基因组或蛋白质组研究、药物筛选、临床诊断以及组合化学领域有广泛应用。