电针干预对颅脑损伤大鼠行为学及血清中NSE浓度的影响

Objective: To observe the effects of electroacupuncture therapy on the behavior, brain tissue morphology and the concentration of NSE in serum of rats with traumatic brain injury (TBI). Methods: 40 healthy male SD rats were randomly divided into a blank group, a sham operation group, a model group and an electroacupuncture group according to the random number table method, with 10 rats in each group. The electroacupuncture group was treated with electroacupuncture after modeling ("Baihui, Neiguan, Quchi, Zusanli, Yongquan" points, once a day for 14 days). The behavioral functions of the rats were evaluated before and after modeling, and on the 3rd, 7th and 14th days after treatment respectively. The pathological morphological changes of brain tissue and the concentration of NSE in serum were observed and measured on the 7th and 14th days. Results: After 14 days of treatment, the balance function of the rats in the electroacupuncture group was better than that after modeling in this group (P < 0.05), and better than that of the model group (P < 0.05). The balance functions of the model group after modeling and after 14 days were lower than those of the sham operation group at the same time period respectively (both P < 0.05). After 14 days of treatment, the walking function of the rats in the electroacupuncture group was significantly improved compared with that after modeling (P < 0.05), and better than that of the model group at the same time period (P < 0.05). After 14 days of treatment, the nuclei of nerve cells in the brain of the rats in the electroacupuncture group were clear, the outlines were clear, and a small amount of vacuolar-like changes could be seen. The arrangement form of nerve cells was better than that of the model group (in the model group: most of the cells were arranged in a sieve-like manner, neurons were degenerated, the vacuolar-like changes of the tissue were obvious, and fibrous hyperplasia and scar tissue appeared around the necrosis). After 14 days of treatment, the concentration of NSE in the serum of the rats in the electroacupuncture group was significantly lower than that of the model group (P < 0.05), and lower than the concentration 7 days ago in this group (P < 0.05). Conclusion: Electroacupuncture intervention can effectively improve the behavioral function of TBI rats and has a certain regulatory and protective effect on the cranial nerves of TBI rats.

目的观察电针疗法对颅脑损伤(TBI)大鼠的行为学、脑组织形态学及血清中NSE浓度的影响。方法40只健康雄性SD大鼠用随机数字表法随机分为空白组、假手术组、模型组、电针组,10只/组。电针组造模后电针治疗(“百会、内关、曲池、足三里、涌泉”穴,1次/天,共14天)。分别于造模前、后、治疗后第3天、7天、14天评价大鼠行为学功能,在第7天、14天取材并观测脑组织病理形态学变化及血清中NSE的浓度。结果治疗14天后电针组大鼠平衡功能优于本组造模后(P <0.05),且优于模型组(P <0.05),而造模后及14天后模型组平衡功能,分别低于同时段假手术组(P均<0.05);治疗14天后电针组大鼠行走功能,较造模后明显提高(P <0.05),且优于同时段模型组P <0.05;治疗14天后电针组脑神经细胞胞核清晰、轮廓清楚、可见少量空泡样改变,神经细胞排列形态优于模型组(模型组:细胞大部分呈筛状排列、神经元变性,组织空泡样改变明显,坏死周围出现纤维增生和瘢痕组织);治疗14天后电针组大鼠血清中NSE浓度明显低于模型组(P <0.05),且低于本组7天前浓度(P <0.05)。结论电针干预能有效改善TBI大鼠行为学功能,对TBI大鼠脑神经有一定的调节和保护作用。