Detection and Quantification of Hydrogen Peroxide in Aqueous Solutions Using Chemical Exchange Saturation Transfer.

The development of new analytical methods to accurately quantify hydrogen peroxide is of great interest. In the current study, we developed a new magnetic resonance (MR) method for noninvasively quantifying hydrogen peroxide (H2O2) in aqueous solutions based on chemical exchange saturation transfer (CEST), an emerging MRI contrast mechanism. Our method can detect H2O2 by its specific CEST signal at ~6.2 ppm downfield from water resonance, with more than 1000 times signal amplification compared to the direct NMR detection. To improve the accuracy of quantification, we comprehensively investigated the effects of sample properties on CEST detection, including pH, temperature, and relaxation times. To accelerate the NMR measurement, we implemented an ultrafast Z-spectroscopic (UFZ) CEST method to boost the acquisition speed to 2 s per CEST spectrum. To accurately quantify H2O2 in unknown samples, we also implemented a standard addition method, which eliminated the need for predetermined calibration curves. Our results clearly demonstrate that the presented CEST-based technique is a simple, noninvasive, quick, and accurate method for quantifying H2O2 in aqueous solutions.

开发能够准确定量过氧化氢的新分析方法具有重大意义。在当前的研究中，我们基于化学交换饱和转移（CEST）这一新兴的磁共振成像（MRI）对比机制，开发了一种用于无创定量水溶液中过氧化氢（H₂O₂）的新磁共振（MR）方法。我们的方法能够通过其在水共振峰低场约6.2 ppm处的特定CEST信号检测H₂O₂，与直接的核磁共振（NMR）检测相比，信号放大了1000多倍。为了提高定量的准确性，我们全面研究了样品性质对CEST检测的影响，包括pH值、温度和弛豫时间。为了加快核磁共振测量，我们采用了一种超快Z光谱（UFZ）CEST方法，将每个CEST谱的采集速度提高到2秒。为了准确定量未知样品中的H₂O₂，我们还采用了一种标准加入法，这种方法无需预先确定的校准曲线。我们的研究结果清楚地表明，所提出的基于CEST的技术是一种用于定量水溶液中H₂O₂的简单、无创、快速且准确的方法。