基于磁共振成像研究侯氏黑散对脑缺血大鼠神经血管功能恢复的作用

Objective To study the effect of Houshi Heisan on the neurovascular function of rats with cerebral ischemia by using magnetic resonance imaging technology. Methods The permanent cerebral ischemia model of rats was established by the middle cerebral artery occlusion method. Male SD rats were randomly divided into the sham operation group, the model group, the low-dose Houshi Heisan group (5.25 g/kg), the high-dose Houshi Heisan group (10.50 g/kg) and the Ginkgo biloba extract group (28 mg/kg). The neurological function scores were used to evaluate the degree of neurological function impairment of rats in each group; T2-weighted imaging and T2 relaxation imaging were used to measure the volume of cerebral infarction and the degree of brain tissue damage in rats with cerebral ischemia; arterial spin labeling perfusion weighted imaging and magnetic resonance angiography were used to evaluate the changes in the hemodynamics of the intracranial blood vessels in rats with cerebral ischemia. Results Compared with the model group, the neurological function scores in the high-dose Houshi Heisan group and the Ginkgo biloba extract group decreased on the 5th and 7th days after cerebral ischemia (P < 0.05). Compared with the model group, the volume of cerebral infarction in each drug-administered group was reduced, and the damage to white matter and gray matter of the brain was alleviated (P < 0.05), and the cerebral blood flow and vascular signal intensity in the ischemic hemisphere increased (P < 0.05). Compared with the sham operation group, the cerebral blood flow and vascular signal intensity in the ischemic hemisphere of the model group decreased (P < 0.01). Conclusion Houshi Heisan can reduce brain damage in rats with cerebral ischemia and improve the intracranial hemodynamics, and play a role in protecting and restoring the neurovascular function.

目的利用磁共振成像技术研究侯氏黑散对脑缺血大鼠神经血管功能的影响。方法采用大脑中动脉线栓法建立大鼠永久性脑缺血模型。雄性SD大鼠随机分为假手术组、模型组、侯氏黑散低剂量组( 5.25 g /kg)组、侯氏黑散高剂量组( 10.50 g /kg)组和银杏叶提取物组( 28 mg /kg) 。神经功能评分评价各组大鼠神经功能缺损程度; T2加权成像和T2弛豫成像测量脑缺血大鼠脑梗死体积和脑组织损伤程度;动脉自旋标记灌注加权成像和磁共振血管成像评价脑缺血大鼠颅内血管的血流动力学的改变。结果与模型组相比,侯氏黑散高剂量组与银杏叶提取物组神经功能评分在脑缺血后第5、7天降低( P < 0.05) 。与模型组相比,各给药组脑梗死体积缩小,脑白质和灰质损伤减轻( P < 0.05),缺血半球脑血流量和血管信号强度增加( P < 0.05) 。与假手术组相比,模型组缺血半球脑血流量和血管信号强度降低( P < 0.01) 。结论侯氏黑散可以减轻脑缺血大鼠脑损伤和改善颅内血流动力学,起到对神经血管功能的保护和恢复作用。