Control of the blue fluorescent protein with advanced evolutionary pulse shaping.

We demonstrate optical coherent control of the two-photon fluorescence of the blue fluorescent protein (BFP), which is of interest in investigations of protein-protein interactions. In addition to biological relevance, BFP represents an interesting target for coherent control from a chemical perspective due to its many components of highly nonexponential fluorescence decay and low quantum yield resulting from excited state isomerization. Using a genetic algorithm with a multiplicative (rather than ratiometric) fitness parameter, we are able to control the ratio of BFP fluorescence to second harmonic generation without a considerable drop in the maximized signal. The importance of linear chirp and power scaling on the discrimination process is investigated in detail.

我们展示了对蓝色荧光蛋白（BFP）双光子荧光的光学相干控制，这在蛋白质 - 蛋白质相互作用的研究中具有重要意义。除了生物学相关性外，从化学角度来看，由于其高度非指数荧光衰减的许多成分以及激发态异构化导致的低量子产率，BFP是相干控制的一个有趣目标。使用具有乘法（而非比率）适应度参数的遗传算法，我们能够控制BFP荧光与二次谐波产生的比率，而不会使最大化的信号大幅下降。详细研究了线性啁啾和功率缩放对区分过程的重要性。