BHBA对HT22细胞中ACE表达的影响

The ketogenic diet (KD) has been proven to have a neuroprotective effect, but its mechanism is still not clear. In this experiment, the mouse hippocampal neuron HT22 was used as a cell model, and the HT22 cells were treated with 2 mmol/L β-hydroxybutyrate (BHBA). Through fluorescence quantitative PCR, Western blot and signal pathway blocking experiments, the effect of BHBA on the expression of ACE in HT22 cells was observed, and the signal pathways involved in regulation were explored. The results showed that 2 mmol/L BHBA could increase the expression of ACE in HT22 cells by activating the GPR109A receptor and ERK1/2 signal pathway in HT22 cells.

生酮饮食(KD)已被证实具有神经保护作用,但其机制尚不明确。本试验以小鼠海马神经元HT22为细胞模型,利用2mmol/Lβ-羟基丁酸酯(BHBA)对HT22细胞进行处理,通过荧光定量PCR,免疫蛋白印迹和信号通路阻断试验,观察BHBA对HT22细胞ACE表达的影响,并探讨参与调控的信号通路。结果表明,2mmol/L BHBA可通过激活HT22细胞内GPR109A受体和ERK1/2信号通路进而增加HT22细胞中ACE的表达。