Role of TAFII-17, a VDR binding protein, in the increased osteoclast formation in Paget's disease

In contrast to normal OCL precursors, pagetic OCL precursors express MVNP and form OCL at physiologic concentrations of 1,25(OH)(2)D-3, as do normal OCL precursors transfected with the MVNP gene. Using a GST-VDR chimeric protein, we identified TAF(II)-17 as VDR binding protein expressed by pagetic OCL precursors and MVNP transduced normal OCL precursors. TAF(II)-17 was in part responsible for the increased 1,25(OH)(2)D-3 responsivity of pagetic OCL precursors.

与正常的破骨细胞（OCL）前体细胞相比，畸形性骨炎（Paget病）的OCL前体细胞表达麻疹病毒核蛋白（MVNP），并且在生理浓度的1,25(OH)(2)D - 3下形成OCL，如同转染了MVNP基因的正常OCL前体细胞一样。利用谷胱甘肽S - 转移酶 - 维生素D受体（GST - VDR）嵌合蛋白，我们鉴定出TAF(II) - 17是由畸形性骨炎的OCL前体细胞和MVNP转导的正常OCL前体细胞所表达的维生素D受体结合蛋白。TAF(II) - 17在一定程度上导致了畸形性骨炎的OCL前体细胞对1,25(OH)(2)D - 3反应性的增加。