Identification of proteins of Pro pionibacterium acnes for use as vaccine candidates to prevent infection by the pig pathogen Actinobacillus pleuropneumoniae

Actinobacillus pleuropneumoniae is the causative agent of acute and chronic pleuroneumonia that is responsible for substantial morbidity and mortality in the pig industry. New improved vaccines that can protect against all serotypes and prevent colonization are required. In a previous study we showed that whole cells of Propionibacterium acnes protected pigs from A. pleuropneumoniae serotype 1 and 5 and, therefore, the basis for a promising heterologous vaccine. The aim of this study was to identify those protein antigens of P. acnes responsible for protection against A. pleuropneumoniae infection. Six P. acnes protein antigens that were recognized by sera raised against A. pleuropneumoniae were identified by 2-DE and immunoblotting. Recombinant versions of all P. acnes proteins gave partial protection (10-80%) againstA. pleuropneumoniae serotype 1 and/or 5 infection in a mouse challenge model. The best protection (80% serotype 1; 60% serotype 5) was obtained using recombinant P. acnes single-stranded DNA-binding protein. In part, protection against A. pleuropneumoniae infection may be mediated by small peptide sequences present in P. acnes single-stranded DNA-binding protein that are cross-reactive with those present in the A. pleuropneumoniae-specific RTX toxin ApxIV and the zinc-binding protein ZnuA. The results suggest that P. acnes may be a useful vaccine to protect against different serotypes of A. pleuropneumoniae. (C) 2013 Elsevier Ltd. All rights reserved.

胸膜肺炎放线杆菌是急性和慢性胸膜肺炎的病原体，在养猪业中造成了大量的发病和死亡。需要能够预防所有血清型并防止定植的新型改良疫苗。在先前的一项研究中，我们发现痤疮丙酸杆菌全细胞可保护猪免受胸膜肺炎放线杆菌血清型1和5的感染，因此它是一种有前景的异源疫苗的基础。本研究的目的是确定痤疮丙酸杆菌中那些对预防胸膜肺炎放线杆菌感染起保护作用的蛋白质抗原。通过双向电泳和免疫印迹法鉴定出6种被胸膜肺炎放线杆菌免疫血清识别的痤疮丙酸杆菌蛋白质抗原。所有痤疮丙酸杆菌蛋白质的重组形式在小鼠攻毒模型中对胸膜肺炎放线杆菌血清型1和/或5感染提供了部分保护（10 - 80%）。使用重组痤疮丙酸杆菌单链DNA结合蛋白可获得最佳保护效果（对血清型1为80%；对血清型5为60%）。在一定程度上，对胸膜肺炎放线杆菌感染的保护可能是由痤疮丙酸杆菌单链DNA结合蛋白中存在的小肽序列介导的，这些小肽序列与胸膜肺炎放线杆菌特异性RTX毒素ApxIV和锌结合蛋白ZnuA中存在的序列发生交叉反应。结果表明，痤疮丙酸杆菌可能是一种用于预防不同血清型胸膜肺炎放线杆菌的有用疫苗。（C）2013爱思唯尔有限公司。保留所有权利。