Biodegradation of p-nitrophenol by engineered strain.

p-Nitrophenol (PNP) is an important environmental pollutant and can causes significant environmental and health risks. Compared with the traditional methods, biodegradation is a useful one to completely remove the harmful pollutants from the environment. Here, an engineered strain was first constructed by introducing PNP biodegradation pathway via the hydroquinone (HQ) pathway into Escherichia coli. In the engineered strain BL-PNP, PNP was completely degraded to β-ketoadipate and subsequently enter the metabolites of multiple anabolic pathways. The high tolerance and rapid degradation ability to PNP enable the engineered strain to have the potential to degrade toxic substances. The engineered strain created in this study can be used as a functional strain for bioremediation of PNP and potential toxic intermediates, and the method of assembling aromatic hydrocarbons metabolic pathway can be used to eradicate nitroaromatic pollutants in the environment. The online version contains supplementary material available at 10.1186/s13568-021-01284-8.

对硝基苯酚（PNP）是一种重要的环境污染物，会造成重大的环境和健康风险。与传统方法相比，生物降解是一种将有害污染物从环境中彻底去除的有效方法。在此，通过将经由对苯二酚（HQ）途径的PNP生物降解途径引入大肠杆菌，首次构建了一种工程菌株。在工程菌株BL - PNP中，PNP被完全降解为β - 酮己二酸，并随后进入多种合成代谢途径的代谢产物中。对PNP的高耐受性和快速降解能力使该工程菌株具有降解有毒物质的潜力。本研究中构建的工程菌株可用作对PNP及潜在有毒中间产物进行生物修复的功能菌株，并且组装芳香烃代谢途径的方法可用于消除环境中的硝基芳香族污染物。 网络版包含补充材料，可在10.1186/s13568 - 2021 - 01284 - 8获取。