CALCB rs3829222 T/T Genotype and Low Expression of CALCB Are High-Risk Factors for Adenoid Cystic Carcinoma of Salivary Gland.

To investigate the relationship between polymorphisms of calcitonin-related peptide gene II (beta-calcitonin gene-related peptide (βCGRP), CALCB) and serum CGRP levels in salivary adenoid cystic carcinoma. Using the polymerase chain reaction (PCR) technique, the full-length amplification and genotype analysis of CALCB genes were performed in 39 patients with adenoid cystic carcinoma of salivary gland and 158 normal controls. The gene frequencies of major genotype of CALCB in adenoid cystic carcinoma of salivary gland and normal control group were analyzed. Enzyme-linked immunosorbent assay (ELISA) was used to evaluate serum calcitonin gene-related peptide (CGRP) and its concentration of alpha and beta subtypes. Univariate logistic regression analysis showed that the CALCB rs2839222 T/T genotype was closely related to the occurrence of salivary adenoid cystic carcinoma, with a correlation coefficient of 3.89. The serum CGRP concentration in the salivary adenoid cystic carcinoma group was 1.56 times that of the normal control group. The αCGRP subtype was significant, which was 3.02 times that of the normal control. The polymorphism of βCGRP gene is associated with genetic susceptibility to salivary adenoid cystic carcinoma, and serum CGRP and βCGRP can be used as novel markers of salivary adenoid cystic carcinoma.

探讨降钙素相关肽基因II（β - 降钙素基因相关肽（βCGRP），CALCB）多态性与唾液腺腺样囊性癌血清CGRP水平之间的关系。 采用聚合酶链反应（PCR）技术，对39例唾液腺腺样囊性癌患者和158例正常对照者进行CALCB基因的全长扩增和基因型分析。分析唾液腺腺样囊性癌组和正常对照组中CALCB主要基因型的基因频率。采用酶联免疫吸附测定（ELISA）评估血清降钙素基因相关肽（CGRP）及其α和β亚型的浓度。 单因素逻辑回归分析显示，CALCB rs2839222 T/T基因型与唾液腺腺样囊性癌的发生密切相关，相关系数为3.89。 唾液腺腺样囊性癌组血清CGRP浓度是正常对照组的1.56倍。αCGRP亚型差异显著，是正常对照组的3.02倍。βCGRP基因多态性与唾液腺腺样囊性癌的遗传易感性相关，血清CGRP和βCGRP可作为唾液腺腺样囊性癌的新型标志物。