纳米流式检测技术的研发、应用及前景

In response to the increasing demand for nanoparticle characterization at the single-particle level in fields such as life sciences, biomedicine, and nanotechnology, our research group has combined Rayleigh scattering and sheath-flow single-molecule fluorescence detection technology and successfully developed nano-flow cytometry (nFCM), enabling highly sensitive, highly selective, and high-throughput detection of the particle size and its distribution, particle concentration, and biochemical properties of single nanoparticles (7 - 500 nm) as well as natural biological nanoparticles such as extracellular vesicles, viruses, bacteria, and subcellular organelles. This article discusses the importance and challenges of nFCM research and development and how to achieve ultrasensitive detection, reviews a series of new biochemical analysis techniques and methods developed based on this technology, and looks forward to the application prospects of nFCM.

针对生命科学、生物医药、纳米科技等领域日益增长的单粒子水平纳米颗粒表征需求,本课题组将瑞利散射和鞘流单分子荧光检测技术结合,成功研发了纳米流式检测技术(nano-flow cytometry,nFCM),实现单个纳米颗粒(7~500 nm)以及细胞外囊泡、病毒、细菌、亚细胞器等天然生物纳米颗粒的粒径及其分布、颗粒浓度和生物化学性状的高灵敏、高选择性、高通量检测.本文对nFCM研发的重要性、挑战及如何实现超灵敏检测进行探讨,对基于该技术所发展的一系列生化分析新技术和方法进行综述,并对nFCM的应用前景进行展望.