Isolation and characterization of Pseudomonas sp strain ONBA-17 degrading o-nitriobenzaldehyde

Aerobic bacteria degrading o-nitrobenzaldehyde (ONBA) were isolated from activated sludges. One of the isolates, ONBA-17, was identified as Pseudomonas sp. The isolate could grow on ONBA as its sole source of carbon and nitrogen. Further studies demonstrated that the strain was a moderately halophilic bacterium and capable of degrading benzoic acid, 2-nitrophenol, 2-aminophenol, 4-hydroxybenzoic acid, and 4-dimetylaminobenzaldehyde. It could completely degrade 100 mg L-1 ONBA at a range of pH 6-8 in 48 h at 30 degrees C, and up to 400 mg L-1 after 288 h. The strain showed potential to be a good candidate for biotreatment of industrial wastewaters containing ONBA due to its salt-tolerance ability, multiresistance to some heavy metals and antibiotics, and the abilities of degradation of aromatic compounds. These findings may help in developing a process for ONBA-containing industrial wastewater treatment.

从活性污泥中分离出可降解邻硝基苯甲醛（ONBA）的好氧细菌。其中一株分离菌ONBA - 17被鉴定为假单胞菌属。该分离菌能够以ONBA作为唯一的碳源和氮源生长。进一步研究表明，该菌株是一种中度嗜盐细菌，能够降解苯甲酸、2 - 硝基苯酚、2 - 氨基苯酚、4 - 羟基苯甲酸和4 - 二甲基氨基苯甲醛。它在30℃、pH 6 - 8的条件下，能在48小时内完全降解100 mg/L的ONBA，在288小时后可降解高达400 mg/L的ONBA。由于该菌株具有耐盐能力、对某些重金属和抗生素的多重抗性以及降解芳香族化合物的能力，它有潜力成为含ONBA工业废水生物处理的良好候选菌株。这些发现可能有助于开发一种处理含ONBA工业废水的工艺。