Bioactive compounds from high altitude lake <em>Arthrospira platensis</em> HANL01: Antioxidant property, thermal stability and antibacterial assessment against multiple antibiotics resistant bacteria

Growing concerns about antibiotic resistance in bacteria and the pursuit of novel compounds and drug candidates are significant strategies for its preparedness. The study aimed to explore Arthrospira sp. isolated from high-altitude lake water for antioxidant potential, thermal stability, and bactericidal action against various bacteria that are resistant to antibiotics. % DPPH free radical scavenging activity was recorded in the range of 15.23 ± 0.38 to 89.21 ± 0.14. Column-purified MeOH fraction and TLC fraction showed significant antibacterial potential. Crude extracts were found stable at 25 °C even after 63 days of storage. Based on the GC–MS profiling, pentadecanoic acid was reported as the primary constituent, as the spectra of this bioactive compounds (11.13 %) were considered as the highest peaks with the highest impact (9.447). The study's findings suggested that high-altitude lake A. platensis is an excellent source of antioxidant and antibacterial substances and might be employed for further drug development research, particularly against multiple antibiotic-resistant bacteria.

对细菌抗生素耐药性的日益关注以及对新型化合物和候选药物的追求是应对这一问题的重要策略。本研究旨在探究从高海拔湖水分离出的节旋藻（Arthrospira sp.）的抗氧化潜力、热稳定性以及对多种抗生素耐药细菌的杀菌作用。DPPH自由基清除活性记录在15.23 ± 0.38到89.21 ± 0.14范围内。柱纯化的甲醇组分和薄层色谱（TLC）组分显示出显著的抗菌潜力。粗提物在25°C下储存63天后仍保持稳定。基于气相色谱 - 质谱（GC - MS）分析，十五烷酸被报告为主要成分，因为这种生物活性化合物（11.13%）的光谱被视为具有最高影响（9.447）的最高峰。该研究结果表明，高海拔湖泊中的钝顶节旋藻（A. platensis）是抗氧化和抗菌物质的优质来源，可能被用于进一步的药物开发研究，特别是针对多重耐药细菌。