黑花生衣原花色素和花色苷的提取工艺研究

In order to simultaneously increase the yields of proanthocyanidins and anthocyanins in black peanut skins, single-factor experiments and response surface analysis experiments were used to optimize the extraction process of proanthocyanidins and anthocyanins in black peanut skins. Based on the single-factor experiments, with the pH value fixed at 3 and the extraction time at 120 min, the liquid-to-solid ratio, ethanol volume fraction, and extraction temperature were selected to design response surface experiments to investigate the effects of each independent variable and its interaction on the yields of proanthocyanidins and anthocyanins. The optimal extraction parameters obtained were: a liquid-to-solid ratio of 52∶1 (mL∶g), an ethanol volume fraction of 48%, and an extraction temperature of 44 °C. Under these conditions, the extraction rates of proanthocyanidins and anthocyanins in black peanut skins were 13.769 mg/100 mg and 0.660 mg/100 mg, respectively.

为了同时提高黑花生衣原花色素和花色苷的得率，采用单因素试验和响应面分析试验对黑花生衣原花色素和花色苷的提取工艺进行优化。 在单因素试验的基础上，固定pH值为3、 提取时间120 min，选取液料比、 乙醇体积分数和提取温度设计响应面试验，考察各自变量及其交互作用对原花色素和花色苷得率的影响。获得最佳提取参数为：液料比52∶1 （ mL∶g）、 乙醇体积分数48%、 提取温度44 ℃。此条件下黑花生衣中原花色素和花色苷的提取率分别为13.769 mg/100 mg、 0.660 mg/100 mg。