Growth Cone Collapse and Neurite Retractions: An Approach to Examine X-irradiation Affects on Neuron Cells

The growth cone is a structure at the terminal of a neurite that plays an important role in the growth of the neurite. The growth cone collapse assay is considered to be a useful method to quantify the effects of various factors on nerve tissue. Here, we investigated the effect of x-irradiation on growth cones and neurites and also the comparative radiosensitivity of different neurons. Dorsal root ganglia and sympathetic chain ganglion were isolated from day-8 and -16 chick embryos and cultured for 20 h. Neurons were then exposed to x-irradiation and morphological changes were quantitatively evaluated by growth cone collapse assay. Cell viability was examined using TUNEL and WST-1 assays. The results showed that radiation induced growth cone collapse and neurite retraction in a time- and exposure-responsive manner. Growth cone collapse, apoptosis and WST-1 assays showed that no significant difference between the neurons throughout the study period (p >= 0.5) after irradiation. Both types of day-8 neurons were more radiosensitive than day-16 neurons (p

生长锥是神经突末端的一种结构，在神经突的生长中起着重要作用。生长锥塌陷试验被认为是一种量化各种因素对神经组织影响的有用方法。在此，我们研究了X射线照射对生长锥和神经突的影响，以及不同神经元的相对放射敏感性。从第8天和第16天的鸡胚中分离出背根神经节和交感链神经节，并培养20小时。然后将神经元暴露于X射线照射下，并通过生长锥塌陷试验对形态变化进行定量评估。使用TUNEL和WST - 1试验检测细胞活力。结果表明，辐射以时间和照射剂量相关的方式诱导生长锥塌陷和神经突回缩。生长锥塌陷、细胞凋亡和WST - 1试验表明，在照射后的整个研究期间，神经元之间无显著差异（p≥0.5）。第8天的两种神经元都比第16天的神经元对辐射更敏感（p