Investigation on Ruthenium(II) Bipyridine/AgIII Complexes Chemiluminescence System and Its Application for Sensitive Norfloxacin and Ofloxacin Detection

The reaction mechanism of Ag-III complex ([Ag(HIO6)(2)](5-)) with ruthenium(II) bipyridine (Ru(bpy)(3)(2+)) in acid medium was investigated. A novel flow injection chemiluminescence (CL) analysis method was developed for the detection of norfloxacin (NFLX) and ofloxacin (OFLX) in commercial drug, milk and human urine samples based on Ag-III-ruthenium(II) bipyridine in acidic solution. Under optimal conditions, CL intensities were proportional to drug concentrations in the range of 1.6 x 10(-9) to 8 x 10(-6) g mL(-1) for NFLX and 1.0 x 10(-9) to 4 x 10(-6) g mL(-1) for OFLX. The detection limit was 1.0 x 10(-9) g mL(-1) for NFLX and 0.8 x 10(-9) g mL(-1) for OFLX. On the basis of the CL, UV and fluorescence spectra, possible reaction mechanisms were proposed.

研究了酸性介质中Ag - III配合物（[Ag(HIO₆)(₂)](⁵⁻)）与钌（II）联吡啶（Ru(bpy)₃²⁺）的反应机理。基于酸性溶液中的Ag - III - 钌（II）联吡啶，开发了一种新型流动注射化学发光（CL）分析方法，用于检测市售药品、牛奶和人尿液样本中的诺氟沙星（NFLX）和氧氟沙星（OFLX）。在最佳条件下，对于NFLX，化学发光强度与药物浓度在1.6×10⁻⁹至8×10⁻⁶ g/mL范围内呈正比；对于OFLX，在1.0×10⁻⁹至4×10⁻⁶ g/mL范围内呈正比。NFLX的检测限为1.0×10⁻⁹ g/mL，OFLX的检测限为0.8×10⁻⁹ g/mL。根据化学发光、紫外和荧光光谱，提出了可能的反应机理。