Association of the Genotypes of Angiotensin Converting Enzyme with the Type 2 Diabetes Mellitus in Khuzestan Province, Iran

Background: The impact of angiotensin converting enzyme (ACE) gene Insertion/deletion (I/D) polymorphism on etiology of type 2 of diabetes mellitus (T2DM) is well established. Reports show that unlike the II or T2DM genotypes the DD genotype of ACE is correlated with high risk of T2DM.In the present study the relationship between ACE gene polymorphism with the T2DM pathogenesis has been investigated in a population living in Khuzestan Province, Iran. Methods: This case-control study performed on diabetic patients in Ahvaz Province, 99 cases and 100 normal individuals were enrolled in this study. T2DM patients were selected according to the WHO criteria. Blood sample was collected from each patient; DNA was extracted and subjected to PCR amplification using specific primers to iden tify different ACE genotypes (II, ID and DD). Results:The chi-square analysis showed that lower frequency of DD genotype in patients is associated with increased risk of T2DM (Odds ratio= 0.273, P=0.004). However in patients suffering from T2DM the ID and II genotypes were significantly increased. Conclusions: It appears that in T2DM a decrease in DD genotype of ACE and increases in DI and II genotypes is associated with changes in fasting blood sugar, triglyceride, total cholesterol; and blood pressure. These data may suggest that the DD genotype of ACE is associated with T2DM in Khuzestan Province.

背景：血管紧张素转换酶（ACE）基因插入/缺失（I/D）多态性对2型糖尿病（T2DM）病因的影响已得到充分证实。报告显示，与II型或其他T2DM相关基因型不同，ACE的DD基因型与T2DM的高风险相关。在本研究中，对伊朗胡齐斯坦省的一个人群进行了ACE基因多态性与T2DM发病机制之间关系的研究。 方法：这项病例对照研究在阿瓦士省的糖尿病患者中进行，99例患者和100名正常个体参与了本研究。T2DM患者是根据世界卫生组织的标准选取的。从每位患者采集血液样本；提取DNA，并使用特异性引物进行聚合酶链反应（PCR）扩增，以识别不同的ACE基因型（II、ID和DD）。 结果：卡方分析表明，患者中DD基因型频率较低与T2DM风险增加相关（优势比 = 0.273，P = 0.004）。然而，在患有T2DM的患者中，ID和II基因型显著增加。 结论：在T2DM中，ACE的DD基因型减少以及DI和II基因型增加似乎与空腹血糖、甘油三酯、总胆固醇和血压的变化相关。这些数据可能表明，在胡齐斯坦省，ACE的DD基因型与T2DM相关。