Single cell differential impedance spectroscopy analys using high density hydrodynamic cell trapping arrays.

We present a novel device for performing differential impedance spectroscopic analysis of single cells hydrodynamically captured in an array of trapping sites. The method combines the advantages offered by impedimetric analysis used for flow cytometry with the ability to capture multiple single cells and perform long-term transient and steady state analysis. We have tested the device by capturing single polystyrene beads and HeLa cells and measuring differential impedance responses.

我们提出了一种新型装置，用于对在一系列捕获位点中流体动力学捕获的单个细胞进行差分阻抗光谱分析。该方法将用于流式细胞术的阻抗分析所提供的优势与捕获多个单个细胞以及进行长期瞬态和稳态分析的能力相结合。我们通过捕获单个聚苯乙烯珠子和海拉细胞并测量差分阻抗响应来测试了该装置。