肌动蛋白亚型在巨噬细胞吞噬脑膜炎大肠杆菌中的作用

This study used the induced THP - 1 monocyte cell line as a model cell of macrophages, identified the actin subtypes expressed in macrophages, and found that β - actin and γ - actin were expressed in macrophages; then, using confocal microscopic imaging, it was found that both β - actin and γ - actin showed different degrees of aggregation when macrophages were infected by Escherichia coli meningitis; further, using RNA interference technology, the specific down - regulation of β - actin and γ - actin in macrophages was achieved, and it was found that the down - regulation of β - actin and γ - actin alone as well as the combined down - regulation of β - and γ - actin significantly decreased the ability of macrophages to phagocytose Escherichia coli meningitis. These results indicate that different subtypes of actin play an important role in the process of macrophages phagocytosing Escherichia coli meningitis.

该研究利用诱导后的THP-1单核细胞系作为巨噬细胞的模式细胞,鉴定了在巨噬细胞中表达的肌动蛋白亚型,发现β-肌动蛋白和γ-肌动蛋白在巨噬细胞中表达;接着利用共聚焦显微成像发现,β-肌动蛋白和γ-肌动蛋白在巨噬细胞受到脑膜炎大肠杆菌感染时均出现了不同程度的聚集;进一步利用RNA干扰技术实现了对巨噬细胞中的β-肌动蛋白和γ-肌动蛋白的特异性下调,并发现单独下调β-肌动蛋白和γ-肌动蛋白以及联合下调β-和γ-肌动蛋白都使得巨噬细胞吞噬脑膜炎大肠杆菌的能力呈明显下降。这些结果表明,不同亚型的肌动蛋白在巨噬细胞吞噬脑膜炎大肠杆菌过程中发挥着重要作用。