Distribution of microbiota in cervical preneoplasia of racially disparate populations.

Microbiome dysbiosis is an important contributing factor in tumor development and thus may be a risk predictor for human malignancies. In the United States, women with Hispanic/Latina (HIS) and African American (AA) background have a higher incidence of cervical cancer and poorer outcomes than Caucasian American (CA) women. Here, we assessed the distribution pattern of microbiota in cervical intraepithelial neoplasia (CIN) lesions obtained from HIS (n = 12), AA (n = 12), and CA (n = 12) women, who were screened for CC risk assessment. We employed a 16S rRNA gene sequencing approach adapted from the NIH-Human Microbiome Project to identify the microbial niche in all CIN lesions (n = 36). We detected an appreciably decreased abundance of beneficial Lactobacillus in the CIN lesions of the AA and HIS women compared to the CA women. Differential abundance of potentially pathogenic Prevotella, Delftia, Gardnerella, and Fastidiosipila was also evident among the various racial groups. An increased abundance of Micrococcus was also evident in AA and HIS women compared to the CA women. The detection level of Rhizobium was higher among the AA ad CA women compared to the HIS women. In addition to the top 10 microbes, a unique niche of 27 microbes was identified exclusively in women with a histopathological diagnosis of CIN. Among these microbes, a group of 8 microbiota; Rubellimicrobium, Podobacter, Brevibacterium, Paracoccus, Atopobium, Brevundimonous, Comamonous, and Novospingobium was detected only in the CIN lesions obtained from AA and CA women. Microbial dysbiosis in the cervical epithelium represented by an increased ratio of potentially pathogenic to beneficial microbes may be associated with increased CC risk disparities. Developing a race-specific reliable panel of microbial markers could be beneficial for CC risk assessment, disease prevention, and/or therapeutic guidance.

微生物群落失调是肿瘤发展的一个重要促成因素，因此可能是人类恶性肿瘤的风险预测指标。在美国，具有西班牙裔/拉丁裔（HIS）和非裔美国人（AA）背景的女性宫颈癌发病率高于且预后比高加索裔美国人（CA）女性更差。 在此，我们评估了从接受宫颈癌风险评估筛查的西班牙裔（n = 12）、非裔美国人（n = 12）和高加索裔美国人（n = 12）女性获取的宫颈上皮内瘤变（CIN）病变中微生物群落的分布模式。我们采用了一种源自美国国立卫生研究院人类微生物组计划的16S rRNA基因测序方法来识别所有CIN病变（n = 36）中的微生物生态位。 我们发现，与高加索裔美国女性相比，非裔美国和西班牙裔女性的CIN病变中有益的乳酸杆菌数量明显减少。在不同种族群体中，潜在致病性的普雷沃氏菌、戴尔福特菌、加德纳菌和苛求菌的数量差异也很明显。与高加索裔美国女性相比，非裔美国和西班牙裔女性中微球菌的数量也明显增加。根瘤菌的检测水平在非裔美国和高加索裔美国女性中高于西班牙裔女性。除了排名前10的微生物外，在组织病理学诊断为CIN的女性中还专门鉴定出了27种微生物的独特生态位。在这些微生物中，有8种微生物群，即红微菌属、足杆菌属、短杆菌属、副球菌属、阿托波菌属、短波单胞菌属、丛毛单胞菌属和新鞘氨醇杆菌属，仅在从非裔美国和高加索裔美国女性获取的CIN病变中被检测到。 以潜在致病性微生物与有益微生物比例增加为代表的宫颈上皮微生物群落失调可能与宫颈癌风险差异增加有关。开发一种针对特定种族的可靠微生物标记物组合可能有利于宫颈癌风险评估、疾病预防和/或治疗指导。