甘蔗鞭黑粉菌致病力差异菌株转录组分析

In order to explore the differences at the transcriptional level among different pathogenicity strains of Ustilago scitaminea and mine the pathogenicity-related genes, the Illumina sequencing technology was used to construct cDNA libraries for two different pathogenicity strains Ss16 (with strong pathogenicity) and Ss47 (with weak pathogenicity), and transcriptome sequencing was carried out. After quality control, bioinformatics analysis was performed on them. The results showed that a total of 8.31G (Ss16) and 9.88G (Ss47) of analysis data (clean reads) were obtained. A total of 649 significantly (P<0.05) differentially expressed genes were obtained by comparing Ss16 and Ss47, among which 299 genes were up-regulated and 350 genes were down-regulated. The results of GO annotation and KEGG pathway enrichment analysis of differentially expressed genes indicated that KEGG pathways were mostly related to protein synthesis, transport and metabolism of substances. The top several pathways with the most significant enrichment were: starch and sucrose metabolism, ABC transporters, degradation of aromatic compounds, metabolic pathways and purine metabolism.

为探明甘蔗鞭黑粉菌不同致病力菌株在转录水平的差异,挖掘致病相关基因,采用Illumina测序技术对2个不同致病力菌株Ss16(强致病力)和Ss47(弱致病力)构建cDNA文库,进行转录组测序,通过质控后对其进行生物信息学分析。结果显示:共获得8.31G(Ss16)和9.88G(Ss47)的分析数据(clean reads),Ss16和Ss47相比较共获得显著(P<0.05)差异表达基因649个,其中上调基因299个,下调基因350个。差异性表达基因的GO注释及KEGG通路富集分析结果表明,KEGG通路多与物质的蛋白合成、运输和代谢相关,富集最显著的前几条通路分别为:淀粉和蔗糖代谢(starch and sucrose metabolism)、ABC转运蛋白(ABC transporters)、芳香族化合物的降解(degradation of aromatic compounds)、代谢途径(metabolic pathways)和嘌呤代谢(purine metabolism)。