Serological Diagnosis of Dengue Infection in Blood Plasma Using Long-Range Surface Plasmon Waveguides

We present a compact, cost-effective, label-free, real-time biosensor based on long-range surface plasmon polariton (LRSPP) gold (Au) waveguides for the detection of dengue-specific immunoglobulin M (IgM) antibody, and we demonstrate detection in actual patient blood plasma samples. Two surface functionalization approaches are proposed and demonstrated: a dengue virus serotype 2 (DENV-2) functionalized surface to capture dengue-specific IgM antibody in blood plasma and the reverse, a blood plasma functionalized surface to capture DENV-2. The results obtained via these two surface functionalization approaches are comparable to, or of greater quality, than those collected by conventional IgM antibody capture enzyme linked immunosorbent assay (MAC-ELISA). Our second functionalization approach was found to minimize nonspecific binding, thus improving the sensitivity and accuracy of the test. We also demonstrate reuse of the biosensors by regenerating the sensing surface down to the virus (or antibody) level or down to the bare Au.

我们提出了一种基于长程表面等离子体激元极化（LRSPP）金（Au）波导的紧凑、经济高效、无标记的实时生物传感器，用于检测登革热特异性免疫球蛋白M（IgM）抗体，并展示了在实际患者血浆样本中的检测。提出并展示了两种表面功能化方法：一种是登革热病毒血清型2（DENV - 2）功能化表面，用于捕获血浆中的登革热特异性IgM抗体；另一种是相反的，血浆功能化表面用于捕获DENV - 2。通过这两种表面功能化方法获得的结果与传统的IgM抗体捕获酶联免疫吸附测定（MAC - ELISA）所收集的结果相当，甚至质量更好。我们发现第二种功能化方法可将非特异性结合降至最低，从而提高检测的灵敏度和准确性。我们还展示了通过将传感表面再生至病毒（或抗体）水平或至裸金来重复使用生物传感器。