3D Imaging and Quantification of the Integrin at a Single-Cell Base on a Multisignal Nanoprobe and Synchrotron Radiation Soft X-ray Tomography Microscopy

The development of three-dimensional (3D) single-cell imaging and protein quantitative methods can provide more comprehensive information for diagnoses. We report the design and synthesis of a multisignal nanoprobe (AuGdNC@BSA-CV) for single-cell 3D imaging and quantifying the integrin alpha(IIb)beta(3) using correlated synchrotron radiation soft X-ray tomography microscopy and an iterative tomographic algorithm termed equally sloped tomography for the first time. Moreover, on the basis of the Au or Gd content of our nanoprobe, the number of integrin alpha(IIb)beta(3) on a single cell also can be accurately quantified (1.5 x 10(7) per cell) via inductively coupled plasma mass spectrometry.

三维（3D）单细胞成像及蛋白质定量方法的发展可为诊断提供更全面的信息。我们首次报道了一种多信号纳米探针（AuGdNC@BSA - CV）的设计与合成，该探针可用于单细胞3D成像以及使用相关同步辐射软X射线断层扫描显微镜和一种称为等倾斜断层扫描的迭代断层扫描算法对整合素α(IIb)β(3)进行定量。此外，基于我们的纳米探针中的金（Au）或钆（Gd）含量，还可通过电感耦合等离子体质谱法精确量化单个细胞上整合素α(IIb)β(3)的数量（每个细胞1.5×10⁷个）。