Production of human cardiomyocytes based on a scalable stem cell culture platform

Cardiovascular diseases are serious and have become the main cause of death worldwide. Cardiomyocytes.(CMs) derived from human induced pluripotent stem cells (hiPSCs) have been widely used in cardiovascular.disease modeling, heart development research, drug screening and potential cell therapy. Efficient production.of CMs from hiPSCs is crucial for its’ basic research and clinical translation. The three-dimensional (3D).suspension culture system is preferred in CMs culture, as it can achieve large-scale production and obtain.more mature phenotype, but a robust stem cell culture platform remains unsolved. We previously established.a scalable PSC culture platform (termed AIC) that supports PSC passaging at the single-cell level under 3D.suspension conditions. However, the efficiency of hiPSCs derived from the AIC system directed differentiation.into CMs remains unclear. The research aims to explore the differentiation efficiency of hiPSCs derived from.the AIC system.

心血管疾病很严重，已成为全球主要的死亡原因。源自人诱导多能干细胞（hiPSCs）的心肌细胞（CMs）已广泛应用于心血管疾病建模、心脏发育研究、药物筛选以及潜在的细胞治疗。从hiPSCs高效生产心肌细胞对其基础研究和临床转化至关重要。三维（3D）悬浮培养系统在心肌细胞培养中更受青睐，因为它可实现大规模生产并获得更成熟的表型，但一个稳健的干细胞培养平台仍未解决。我们先前建立了一个可扩展的多能干细胞培养平台（称为AIC），该平台支持在3D悬浮条件下对多能干细胞进行单细胞水平的传代。然而，源自AIC系统的hiPSCs定向分化为心肌细胞的效率仍不清楚。本研究旨在探索源自AIC系统的hiPSCs的分化效率。