Recent advances of boronate affinity materials in sample preparation

Boronate affinity materials, as widely used sorbents for the enrichment of cis-diol-containing molecules, have been rapidly developed and increasingly utilized for various applications in recent years. cis-Diol-containing molecules, including saccharides, nucleosides, catecholamines, glycans and glycoproteins/glycopeptides, are major targets in the frontiers of many research areas, such as environmental analysis, the food industry and bioanalysis. As the analysis of these molecules usually suffers from the low abundance of targets and the high abundance of interference, selective enrichment is a fundamental step of sample preparation before analysis. In this review, we survey recent achievements of boronate affinity materials and their applications in sample preparation. We mainly focus on the fundamental considerations of materials as well as important applications in the past 3 years. Particularly, the effects of the substrate structure on the performance of boronate affinity materials, such as binding capacity, affinity, selectivity and working pH, will be discussed. Furthermore, the applications in sample preparation will also be introduced, with a main emphasis on what merits can be provided by boronate affinity materials to overcome the challenges in sample preparation. (C) 2019 Elsevier B.V. All rights reserved.

硼酸亲和材料作为广泛用于富集含顺式二醇分子的吸附剂，近年来得到了迅速发展，并越来越多地应用于各种领域。含顺式二醇的分子，包括糖类、核苷、儿茶酚胺、聚糖以及糖蛋白/糖肽，是环境分析、食品工业和生物分析等许多研究领域前沿的主要研究对象。由于对这些分子的分析通常面临目标物丰度低和干扰物丰度高的问题，选择性富集是分析前样品制备的一个基本步骤。在这篇综述中，我们综述了硼酸亲和材料的最新研究成果及其在样品制备中的应用。我们主要关注材料的基本考量以及过去3年的重要应用。特别是，将讨论基质结构对硼酸亲和材料性能的影响，如结合能力、亲和力、选择性和工作pH值。此外，还将介绍其在样品制备中的应用，主要强调硼酸亲和材料能够提供哪些优势来克服样品制备中的挑战。（C）2019爱思唯尔公司。保留所有权利。