蒙药草乌及其炮制品的六种生物碱类成分血浆中含量测定

Abstract: Objective: To establish an LC - MS method for the determination of six alkaloid components in plasma of Aconitum kusnezoffii Reichb. and its processed products with Terminalia chebula Retz. decoction. Methods: Plasma samples were extracted with 25% ammonia water and ether, and then detected using a ZORbax - Extend - C18 4.6×250 mm 5 - Micron 80A column. The aqueous phase of the mobile phase was a 10 mmol ammonium acetate aqueous solution (adjusted to pH = 9.0 with ammonia water), and the organic phase was acetonitrile. The selected ion detections were m/z 604 (benzoylaconine), m/z 590 (benzoylmesaconine), m/z 574 (benzoylhypaconine), m/z 632 (mesaconitine), m/z 646 (aconitine), m/z 616 (hypaconitine). Results: The linear relationships of the six components in this method were all good. The RSDs of precision, stability and matrix effect were all lower than 10%, and the recoveries ranged from 59.11% to 96.57%. Conclusion: This sample treatment is rapid and simple, and the detection method has high precision. It is suitable for the detection of mesaconitine, hypaconitine, aconitine, benzoylmesaconine, benzoylhypaconine and benzoylaconine in plasma, and provides a reference for the further pharmacokinetic and toxicokinetic studies of Aconitum kusnezoffii Reichb. and Aconitum kusnezoffii Reichb. processed with Terminalia chebula Retz. decoction.

摘要：目的:建立血浆中,草乌及其诃子汤炮制品的6种生物碱类成分LC-MS含量测定的方法。方法:血浆样品用加25%氨水、乙醚萃取后,采用ZORbax-Extend-C18 4.6×250mm 5-Micron 80A柱子检测,流动相水相为10mmol醋酸铵水溶液(氨水调ph=9.0)有机相为乙腈,选择离子检测m/z 604(苯甲酰乌头原碱)、m/z 590(苯甲酰新乌头原碱)、m/z 574(苯甲酰次乌头原碱)m/z 632(新乌头碱)、m/z 646(乌头碱)、m/z 616(次乌头碱)。结果:本方法 6种成分线性关系均良好,精密度、稳定性、基质效应RSD都低于10%,加样回收率为59.11%~96.57%。结论:此样品处理快速简便、检测方法精密度高,适用于血浆中新乌头碱、次乌头碱、乌头碱、苯甲酰新乌头原碱、苯甲酰次乌头原碱、苯甲酰乌头原碱检测,以其为草乌及诃子汤炮制草乌的进一步药代毒代动力学研究提供参考。