Localization of dopamine D1-receptor to A-type horizontal cells in the rabbit retina by single cell RT-PCR

Dopamine is an important neurotransmitter regulating light-dark adaptation in the retina. The effect of dopamine is widespread and dependent on the localization of its receptors. We performed single cell RT-PCR on A-type horizontal cells visually selected from dissociated rabbit retinal neurons. Dopamine D1-receptor mRNA was positively identified. Subsequent sequencing of the fragment showed 82% homology with rat D1 receptor and 87% homology with human D1 receptor. This study supported previous observations that dopamine regulated A-type horizontal cell coupling via D1 receptors at the level of transcription. (C) 2003 Elsevier Ireland Ltd. All rights reserved.

多巴胺是一种在视网膜中调节明适应和暗适应的重要神经递质。多巴胺的作用广泛且取决于其受体的定位。我们对从分离的兔视网膜神经元中目视挑选出的A型水平细胞进行了单细胞逆转录聚合酶链反应（RT - PCR）。多巴胺D1受体的信使核糖核酸（mRNA）得到了阳性鉴定。随后对该片段进行测序，结果显示其与大鼠D1受体有82%的同源性，与人类D1受体有87%的同源性。这项研究支持了先前的观察结果，即多巴胺在转录水平上通过D1受体调节A型水平细胞的耦合。（C）2003爱思唯尔爱尔兰有限公司。保留所有权利。