Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family 'Oxalobacteraceae' isolated from China.

A taxonomic study was performed on strain YIM 31775(T), which was isolated from a soil sample collected from Yunnan Province, China. The isolate was chemo-organotrophic, aerobic and Gram-negative. Cells were short rods and motile, with one or more polar flagella. Growth temperature and pH ranged from 4 to 55 degrees C and 6.5 to 12.0, respectively; the optimum growth temperature and pH were 28-37 degrees C and 7.0-9.0, respectively. Q-8 was the predominant respiratory lipoquinone. The major fatty acids were C(16 : 1)omega7c (42.4 %) and C(16 : 0) (28.1 %). The DNA G + C content was 62.4 +/ -0.3 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain YIM 31775(T) should be placed within the family 'Oxalobacteraceae', in which it formed a distinct lineage. Based on the high 16S rRNA gene sequence divergence and phenotypic characteristics, it is proposed that strain YIM 31775(T) should be classified as representing a novel member of the family 'Oxalobacteraceae', for which the name Naxibacter alkalitolerans gen. nov., sp. nov. is proposed. The type strain is YIM 31775(T) (= CCTCC AA 204003(T) = KCTC 12194(T)).

对菌株YIM 31775(T)进行了分类学研究，该菌株是从中国云南省采集的一份土壤样本中分离得到的。该分离株为化能有机营养型、好氧且革兰氏阴性。细胞为短杆状且能运动，具有一根或多根极生鞭毛。生长温度范围为4 - 55℃，pH范围为6.5 - 12.0；最适生长温度和pH分别为28 - 37℃和7.0 - 9.0。Q - 8是主要的呼吸醌。主要脂肪酸为C(16 : 1)ω7c（42.4%）和C(16 : 0)（28.1%）。DNA G + C含量为62.4 ± 0.3 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株YIM 31775(T)应归属于草酸杆菌科（Oxalobacteraceae），在该科中它形成一个独特的分支。基于较高的16S rRNA基因序列差异和表型特征，建议将菌株YIM 31775(T)分类为草酸杆菌科的一个新成员，提议将其命名为耐碱纳西杆菌（Naxibacter alkalitolerans）新属，新种。模式菌株为YIM 31775(T)（= CCTCC AA 204003(T) = KCTC 12194(T)）