基于核生长动力学的纳米硒-多糖复合物制备过程研究

Selenium–polysaccharide nanoparticles become a research focus for its high biological utility, low toxicity, and noticeable anti-tumor activity. Various sizes and shapes of this composite have different biological activities. The lack of theoretical guidance in the preparation process of selenium–polysaccharide nanoparticles leads to difficulty in obtaining shape uniformity, thereby hindering its usability. To overcome this challenge, this research targeted the growth process of the nucleus of selenium–polysaccharide nanoparticles, then modeling tests in vivo and in vitro were conducted and a precise model defining the correlations between different nanocompounds and their biological activities was set up.Furthermore, in order to realize the directional regulation of the selenium–polysaccharide nanoparticles, the nucleation process of the nanocomposites with high biological activity was studied. Device representation measures, such as a continuous-flow in situ X-ray absorption fine structure (XAFS) spectroscopy for time-resolved studies were used to supervise the small nanocluster nucleation process of the selenium–polysaccharide nanoparticles at the nanometer level to observe its initial nucleation mechanism. Data were then collected and analyzed, and the initial kinetic nucleation model of the composite was established to provide theoretical guidance for directional regulation of its synthesis process, thus laying the foundation for the directional and uniform synthesis of the Selenium-polysaccharide nanoparticles with high biological activity.

纳米硒-多糖复合物因其高生物利用度、低毒性和显著的抗肿瘤活性而成为研究热点。不同尺寸、形貌的纳米硒-多糖复合物所对应的生物活性具有显著差别，而在其制备过程中由于缺乏相应的理论指导依据，往往不能获得均一形态的复合物，成为限制纳米硒-多糖复合物应用的主要瓶颈。针对这一问题，本研究以纳米硒-多糖复合物核生长过程为研究对象，通过体内和体外建模实验，建立不同形态纳米复合物与生物活性之间的精准关联模型，并旨在对高生物活性的纳米复合物的复合物成核过程的解析，实现复合物尺寸、形貌的定向调控。主要采用原位X射线吸收精细结构技术（XAFS）等仪器表征手段，在纳米级尺度上实时监测纳米硒-多糖复合物的成核过程，揭示其成核机制；进而结合统计分析，建立纳米硒-多糖复合物的生长动力学模型，为最终实现高生物活性纳米硒-多糖复合物的定向、均一化合成奠定基础。

纳米硒-多糖复合物因其高生物利用度、低毒性和显著的抗肿瘤活性而成为研究热点。不同尺寸、形貌的纳米硒-多糖复合物所对应的生物活性具有显著差别，而在其制备过程中由于缺乏相应的理论指导依据，往往不能获得均一形态的复合物，成为限制纳米硒-多糖复合物应用的主要瓶颈。针对这一问题，本研究以纳米硒-多糖复合物核生长过程为研究对象，通过体内和体外建模实验，建立不同形态纳米复合物与生物活性之间的精准关联模型，并旨在对高生物活性的纳米复合物的复合物成核过程的解析，实现复合物尺寸、形貌的定向调控。主要采用原位X射线吸收精细结构技术（XAFS）等仪器表征手段，在纳米级尺度上实时监测纳米硒-多糖复合物的成核过程，揭示其成核机制；进而结合统计分析，建立纳米硒-多糖复合物的生长动力学模型，为最终实现高生物活性纳米硒-多糖复合物的定向、均一化合成奠定基础。

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