余甘子中抗烟草花叶病毒活性成分的提取分离与作用机制研究

Systemically bioassay detection indicated that Phyllanthus emblica L. possesses high inhibitory activity against tobacco virus. The continued phytochemical work lead to isolation of Furosin, which was a polyphenol possessed high inhibitory activity against tobacco virus, its inhibitory efficacy is noticeably higher than the antivirus pesticide we used. In this project, activity identification method will be used for isolation, purification, and structure detection of active secondary compounds of this plant. Use Nicotiana glutinosa and Nicotiana tabacum L. val: K326 and as the material, half-leave method, leaf disk method, and disease index survey were used to evaluate the protection and curative activity of compounds isolated, in order to identify its virulence and mechanisms. And then physiological and chemical detection,resistance related genes expression, TMV-CP and TMV-RNA, and Ultramicro pathology structure observation methods were used for the mechanism detection by the comparison of Nicotiana tabacum and TMV. This project is promising and hence merits further studies considering its progress in the intellectual proprietary and property rights for botanical antivirus agents and providing a new method for the integrate management of plant virus.

系统的室内生物测定试验结果表明，余甘子提取物具有显著的抗TMV活性。通过进一步的化学成分研究，从中分离得到的多酚Furosin具有对TMV的体外钝化、抑制侵染和抑制增值活性，且强于阳性对照。本项目将在前期研究基础上，采用活性追踪法，分别对余甘子果实、根及枝叶中抗植物病毒活性物质进行分离、纯化和结构鉴定，明确1-2个高活性成分；进而以半叶枯斑法、叶圆片法和病情指数调查相结合，综合评定分离出的高活性化合物对TMV病毒粒子的体外钝化、保护和治疗作用，以确定其毒力和作用方式；同时，以普通烟和TMV病毒粒子为研究对象，从生理生化指标、抗病相关基因表达、病毒核酸、蛋白及寄主病理结构超微观察等方面探讨其抗病机理。项目研究结果将为创制具有自主知识产权的新型微生物源抗病毒剂奠定基础，为植物病毒病的综合治理提供新的途径。

系统的室内生物测定试验结果表明，余甘子提取物具有显著的抗TMV活性。通过进一步的化学成分研究，从中分离得到的多酚Furosin具有对TMV的体外钝化、抑制侵染和抑制增值活性，且强于阳性对照。本项目将在前期研究基础上，采用活性追踪法，分别对余甘子果实、根及枝叶中抗植物病毒活性物质进行分离、纯化和结构鉴定，明确1-2个高活性成分；进而以半叶枯斑法、叶圆片法和病情指数调查相结合，综合评定分离出的高活性化合物对TMV病毒粒子的体外钝化、保护和治疗作用，以确定其毒力和作用方式；同时，以普通烟和TMV病毒粒子为研究对象，从生理生化指标、抗病相关基因表达、病毒核酸、蛋白及寄主病理结构超微观察等方面探讨其抗病机理。项目研究结果将为创制具有自主知识产权的新型微生物源抗病毒剂奠定基础，为植物病毒病的综合治理提供新的途径。

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