去泛素化对哺乳动物精子顶体素活化、卵母细胞透明带硬化及多精入卵的影响

Mammalian spermatozoa lose their activity in the process of cooling , called" cold shock". This phenomenon leads to death of many spermatozoa or loss of vitality, the mammalian germplasm resources preservation to our country brings great hidden danger. Generally considered, low fertilization rate for "cold shock" sperm is because sperm suffer the injury in the cooling process of low temperature, including "capacitation-like"change,called cryo-capacitation.Capacitationand"capacitation -like"sperm have many similarities, but capacitation sperm can normal fertilization, and" capacitation-like" sperm have low fertility or loss of fertility, the differences has not been reported. Therefore, we mainly study effects of de -ubiquitination on acrosin activation, ZP (zona pellucida ,ZP)hardening and polyspermy, on the basis of this study Through the comparison of" acrosin activation pathway differences of capacitation" and" capacitation-like" sperm , clarify" The reason of low fertility of capacitation-like" sperm , for the improvement of mammalian sperm freezing efficiency and reduce polyspermy provides effective measures. The studies show that after the freezing injury of sperm fertilization has repair mechanisms, but the repair is limited, often resulting in late developmental defects. The completion of this project will provide a theoretical basis for improving preservation of mammalian germplasm resources, reduction of cryoinjury sperm and cure of sterility infertility.

哺乳动物精子在迅速冷却时失去活力，称之为"冷休克"。这种现象导致精子大量死亡或丧失活力，给我国哺乳动物种质资源保存带来巨大的隐患。普遍认为"冷休克"精子受精率下降是因为精子冷却过程中遭受了低温损伤，这种损伤包括"获能样"变化，称之为低温获能。获能与"获能样"精子间有许多相似之处，但获能精子能正常受精，而"获能样"精子受精力低或丧失受精力，产生这种差异的研究未见报道。因此，我们主要研究哺乳动物精子去泛素化对顶素体活化、透明带硬化及多精入卵的影响，在此研究基础上通过比较"获能"与"获能样"精子顶体素活化途径的差异,阐明"获能样"精子受精能力低的原因,为改善哺乳动物精子冷冻效率及降低多精入卵提供有效措施。研究表明尽管冻伤精子受精后有损伤修复机制，但这种修复是有限的，往往导致后期发育的缺陷。本项目的完成将为改进哺乳动物种质资源保存，降低精子低温损伤以及不孕不育治愈等方面提供理论依据。