植物源烟水对丹参次生代谢产物积累的影响及“smoke signals”机制研究

Plant-derived smoke water is produced by combusting plant materials and bubbling smoke through water. The ecological effects of smoke water caused the attention of ecologists. Research abroad is very active while less attention in China. It was found that great changes had taken place in the content and composition of secondary metabolites in Salvia miltiorrhiza which were planted in after "burning" field. Furthermore, it has been confirmed by controlled experiments that smoke water contains chemical signal molecules which could affect the accumulation of secondary metabolites in S. miltiorrhiza. A scientific hypothesis was proposed in this project that plant-derived smoke water could regulate the accumulation of secondary metabolites in S. miltiorrhiza based on “smoke signals” mechanism. Based on the preliminary verification of signal molecule (KAR1) in the smoke water, signal molecules will be separated from smoke water on the basis of “precision discovery”strategy. The effects of smoke water on the secondary metabolites of S. miltiorrhiza will be studied using metabolomics technologies. Then, the signal transduction of secondary metabolism in S. miltiorrhiza regulated by smoke water will be discussed using means of transcriptomics and proteomics. It is hopeful that this project will be the pioneering study of smoke water in resource ecology of traditional Chinese medicine and endow the ancient tradition of "burning" with new ecological connotation.

植物源烟水是指植物燃烧产生的烟溶入水中所得的溶液，烟水的生态学效应引起了生态学界的极大关注，相关研究在国外非常活跃，国内关注较少。申请人发现野外“烧荒”后的过火痕地上种植的丹参植物体内次生代谢产物的含量与组成发生了很大变化，进一步实验证明了烟水中存在调控丹参次生代谢的化学信号分子，由此提出“基于‘smoke signals’机制的植物源烟水调控丹参次生代谢产物积累”的科学假说。本项目将在初步明确烟水中信号分子（丁烯羟酸内酯，KAR1）的基础上，采用“精准”发现策略分离烟水中的信号分子；采用代谢组学的手段揭示信号分子对丹参次生代谢整体轮廓的影响；采用转录组学和蛋白组学的手段揭示信号分子调控丹参次生代谢的信号转导机制。本项目将开拓烟水在我国中药资源生态学领域的研究，赋予“烧荒”这一古老传统以新的生态学内涵。