形变和形状特性对生物粒子惯性迁移行为的影响机理研究

On the basis of our previous studies on inertial focusing mechanisms of rigid polymer microspheres, we propose a novel decoupling study of the inertial migration behaviors of bioparticles with complex features in classic spiral inertial microfluidic devices. First, experiments on migration behaviors of man-made particle models with single controllable features, together with the 3D numerical simulations of inertial flows, will be carried out to probe into the effects of deformation and shape on particle inertial migrations. Then, based on the obtained knowledge on inertial migration, the migration mechanisms of bioparticles with coupled features will be analyzed. Finally, high-throughput and high-sensitivity isolation of malaria-infected red blood cells (miRBCs) from human blood will be conducted according to the deformability difference between iRBCs and healthy RBCs, providing a miniaturized and low-cost tool for the efficient diagnosis of malaria at the early stage of infection.

基于申请课题组前期在刚性聚合物微球惯性聚焦机理方面的研究基础，本项目提出对经典螺旋流道惯性微流控芯片中复杂特性生物粒子的惯性迁移机理进行解耦研究。具体通过实验表征人工制备单特性假体粒子模型的惯性迁移行为，配套开展考虑粒子形变和形状效应的惯性流三维数值模拟，全面掌握单体形变和形状特性对Dean耦合惯性流中粒子迁移行为的影响机理。在此基础上，基于掌握的迁移机理知识库，解析形变和非球形状耦合下生物粒子惯性迁移行为的形成机制；并最终依据形变性差异，指导实现血液中感染疟疾红细胞的高通量、精确分选，为疟疾的早期诊断提供重要微型化工具。