低毒纳米晶电化学发光与信号放大用于乳腺癌细胞和相关microRNA的高灵敏检测与成像分析

Breast cancer is one of the women’s most common malignancy, it is of crucial significance for early diagnosis of breast cancer to develop new, rapid, highly sensitive and selective method for detecting breast cancer cells and related miRNA. In this project, we intend to detect breast cancer cells and miRNA by using highly sensitive electrochemiluminescence of low-toxic nanocrystals. We would prepare novel, low-toxic nanocrystals and nanocomposites with excellent ECL properties, and establish new techniques to enhance ECL of nanocrystals. Based on specific biological recognition, combining nano-amplification and surface resonance-enhancing ECL effects between gold nanoparticles and nanocrystals, we would develop novel nanocrystal biological probe with amplified ECL signal which can specifically recognize cancer cells and miRNA. We would build nano-amplified biosensing interface by using graphene oxide (GO), silver nano-cluster and PAMAM materials etc., and develop new highly sensitive ECL biosensing strategy to detect cancer cells and miRNA by using multiple enzyme-aided cyclic amplification signal techniques with sensitive nanocrystal ECL biological probe. We would develop new nanocrystal ECL imaging analysis techniques, and achieve in situ ECL imaging and visual analysis of breast cancer cells and related miRNA, which would provide more accurate detection technology and evidence for early diagnosis of breast cancer.

乳腺癌是女性最常见的恶性肿瘤之一，发展快速、高灵敏、高选择性的检测癌细胞及相关miRNA的新方法对乳腺癌早期诊断具有极其重要的意义。本项目以乳腺癌细胞及相关miRNA为检测对象，以高灵敏的纳米晶电化学发光（ECL）为检测技术，拟开发制备新型、低毒、高ECL性能的纳米晶及其复合物，建立增强纳米晶ECL的新技术；基于特异性的生物识别反应，结合纳米放大效应、金属与纳米晶之间的表面共振增强ECL效应，研制新型、具有放大信号的、特异性识别癌细胞或miRNA的纳米晶ECL生物探针；利用氧化石墨烯、银纳米簇、PAMAM等材料构建具有纳米放大效应的生物传感界面，将多种酶循环放大信号的技术与纳米晶ECL生物探针相结合，发展高灵敏检测乳腺癌细胞及miRNA的ECL生物传感新策略；建立纳米晶ECL成像分析新技术，实现乳腺癌细胞及miRNA的ECL原位成像和可视化分析，为乳腺癌早期诊断提供更为准确的检测技术和依据