基于“无形之痰”理论探讨健脾祛湿方药二陈汤对血管周脂肪功能的影响及其作用机制

In the theory of Traditional Chinese Medicine, having large amounts of high-calorie food could damage the clearing function of Pi and induce the occurrence of “Tan Shi” in vivo. The access food could be transformed into triglycerides. As a form of invisible phlegm, triglycerides accumulate in perivascular adipose tissue and induces atherosclerosis. Based on our previous investigations, we speculate that Jian Pi Qu Shi formula Er Chen decoction may improve the function of perivascular adipose tissue via skeletal muscle and adipose tissue clearing the invisible phlegm. In order to investigate the mechanism by which Er Chen decoction protects against perivascular adipose tissue dysfunction, this study firstly investigates the effects of Er Chen decoction on the anti-contractile function of perivascular adipose tissue and the triglyceride clearance ability of skeletal muscle and adipose tissue by using various molecular biological techniques in high-fat diet fed rats. Secondly, in C2C12 skeletal muscle cells and 3T3-L1 adipocytes by using Elisa, qPCR, Western-blot and siRNA we investigate the effects of Er Chen decoction containing serum on the process of triglyceride clearance and the involvement of AMPK pathway in this process. This study would be able to develop the scientific concept of invisible phlegm and provide a novel thinking for dealing with atherosclerosis.

中医认为过食“肥甘厚腻”会损伤脾的运化功能而导致体内形成“痰湿”。过剩的食物在体内转化为甘油三酯，其作为“无形之痰”累积于血管周脂肪而诱发动脉粥样硬化。在前期研究的基础上，我们认为健脾祛湿方药二陈汤通过骨骼肌、脂肪组织运化 “无形之痰”而改善血管周脂肪功能。为研究二陈汤抗血管周脂肪功能紊乱的机制，本项目首先在高脂饮食的大鼠模型中，应用多种分子生物学技术研究二陈汤对血管周脂肪抗血管收缩功能及对骨骼肌、脂肪组织“运化”甘油三酯的影响。其次，在C2C12骨骼肌细胞、3T3-L1脂肪细胞中，应用Elisa、qPCR、Western-blot、siRNA等技术研究二陈汤含药血清对骨骼肌细胞、脂肪细胞“运化”甘油三酯的影响以及AMPK信号通路在其中的作用。本项目将丰富“无形之痰”的科学内涵，为动脉粥样硬化的防治提供新思路。