水热耦合机械预处理木质纤维吸附纤维素酶行为及调控机制的研究

Pretreatment is the key for the conversion and utilization of bioresources. The combination of hydrothermal pretreatment and mechanical refining would fully play their respective advantages in the pretreatment of lignocellulosic biomass. The combined pretreatment can not only extract valuable components (e.g., hemicelluloses) from biomass, reduce energy consumption of mechanical refining, separate the fibers, but also can change the physical and chemical properties of lignocellulosic biomass, improve the accessibility of cellulose to enzymes, thereby enhance the enzymatic hydrolysis efficiency of lignocellulosic biomass to provide fermentable sugars for biofuels' production. This present project focuses on the absorption behavior of cellulase onto pretreated lignocellulosic biomass by combination of hydrothermal pretreatment and mechanical refining and the regulation mechanism of the whole process. The research work will be conducted by combing two paths of theoretical research and process optimization, and using multi-angle and multi-scale methods to analysis and characterize. The interaction and synergy between the hydrothermal pretreatment and mechanical refining, the effect of the combined pretreatment on the separation of fibers and fibers’ characteristics, as well as the absorption behavior of cellulase onto the obtained fibers substrates were studied comprehensively and systematically. The influence and regulation mechanism of the combined pretreatment on the subsequent enzymatic hydrolysis of lignocellulosic biomass would be established finally. The research of this project will provide theoretical basis for the improvement and optimization of pretreatment technology, and promote the efficient integration of biorefinery and pulp & paper industry.

预处理是实现生物质资源高效转化和利用的关键。水热耦合机械预处理能够充分发挥各自的优势，既能提取部分半纤维素等有价值的组分，降低机械处理能耗，又能实现木质纤维的分离，改变木质纤维的物理化学特性，提高纤维素的可及度，从而强化酶水解效率为生产生物能源提供可发酵糖。本项目围绕“水热耦合机械预处理木质纤维吸附纤维素酶行为及调控机制”开展研究，结合理论研究和工艺优化两条路径，借助多角度、多尺度的分析和表征，全面、系统地研究水热耦合机械预处理间的相互影响和增效、预处理对木质纤维的分离及纤维特性的影响以及木质纤维底物对纤维素酶的吸附行为，以期明确水热耦合机械预处理对酶水解的影响机理和调控机制，实现生物质资源的高效转化和利用。该项目的研究将为进一步丰富生物质资源的预处理提供技术支持和理论依据，促进生物质精炼与制浆造纸工业的融合。

预处理是实现生物质资源高效转化和利用的关键。水热耦合机械预处理能够充分发挥各自的优势，既能提取部分半纤维素等有价值的组分，降低机械处理能耗，又能实现木质纤维的分离，改变木质纤维的物理化学特性，提高纤维素的可及度，从而强化酶水解效率为生产生物能源提供可发酵糖。本项目围绕“水热耦合机械预处理木质纤维吸附纤维素酶行为及调控机制”开展研究，结合理论研究和工艺优化两条路径，借助多角度、多尺度的分析和表征，全面、系统地研究水热耦合机械预处理间的相互影响和增效、预处理对木质纤维的分离及纤维特性的影响以及木质纤维底物对纤维素酶的吸附行为，以期明确水热耦合机械预处理对酶水解的影响机理和调控机制，实现生物质资源的高效转化和利用。该项目的研究将为进一步丰富生物质资源的预处理提供技术支持和理论依据，促进生物质精炼与制浆造纸工业的融合。

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