欧当归（Leviasticum officinale Koch）杀粘虫卵活性成分及其作用机理研究

Leviasticum officinale Koch is a perennial herbaceous plant belonging to Levisticum Hill of Umbelliferae. Project team previous research shows that the petroleum ether extract of L.officinale Koch showed the highest activity against ovum of Mythimna separata (Walker) ,but active compounds of the extract and action mechanism against the ovum have not yet deeply expanded. Based on the result of the previous researches, using the ovum as biological material in the project, the active compounds are separated and purified by combination the leaching ovum method ,the activity tracking method , microdetermination method and chemical separating method to obtain lead compounds. At the same time ,the structure of lead compounds are identified by spectroscopic means.Then, the action mechanism of the lead compounds against the ovum are studied by combination of the poisoned symptoms ,histology observationand ,method of physiological and biochemical analysis. These results can provide a research foundation for the further synthetic and the optimal design of strong active compounds.

欧当归(Leviasticum officinale Koch)系伞形科欧当归属多年生草本植物。项目组前期研究发现，欧当归提取物具有很强的杀粘虫卵活性，但对其杀卵活性成分及作用机理尚未开展系统研究。本项目在前期工作的基础上，以粘虫卵为试材，采用活性示踪法、微量生测筛选法和化学分离手段相结合的方法进行活性物质的进一步分离纯化，筛选出杀卵活性最高化合物；同时用波谱学方法完成化合物的结构分析与鉴定；通过对卵壳的症状学观察、卵组织学和组织化学分析、胚胎发育过程的细胞学观察、卵的生化指标分析等，开展活性化合物的杀卵机理研究，明确其杀卵作用机理。其研究结果可为下一步合成及结构优化获得杀卵活性更高的化合物提供研究基础。