黄土高原旱地小麦根际AMF多样性与氮肥运筹的协同机制研究

There are significant benefits in wheat yield achieveing, fertilizer policy and preventing pollution from environment and freshwater through fertilizer applying properly via enhancing AMF function in N uptaking.This project is mainly engaged in the relationship between AMF biodiversity and nitrogen use efficiency (NUE) based on different cultivation techniques for wheat in dryland condition of Loess plateau. Synergy ascension effect mechanism should be detected by analyzing component of AMF biodiversity according the pattern of N supplying and translocation from AMF to host plants．The roles of AMF in the N allocation in host plants and the related ecological significance at community and ecosystem levels will be elucidated，this can benefit to create a new wheat cultivation techniques and some issues to be further elucidated on the N metabolism in the wheat root-AMF symbiote.

科学施氮以及提升菌根对土壤氮素的转运对于确立黄土高原旱地小麦土壤培肥目标、提高小麦产量、减缓环境与水质污染意义重大。本项目以旱地小麦根系及根际土壤为研究对象，拟研究不同栽培措施结合施氮条件下，土壤氮素转运、氮素组分残留与根际AMF(丛枝菌根真菌)多样性变化的关系以及双向调节机制。借助山西农业大学黄土高原旱地小麦定位试验站（1978-至今，山西闻喜）这一平台，立足旱地小麦不同栽培措施及施氮梯度的周年试验，通过对小麦不同生育期土壤氮素组分及氮素利用效率的测定、并采用目前最为先进的宏基因组测序技术对根际AMF多样性进行分析，研究氮肥运筹与AMF多样性变化的关系，以探明AMF多样性的菌根群落构成与土壤氮素利用效率的协同提升效应机理，充分挖掘旱地小麦高效利用氮素资源的土壤生物学潜力，为建立黄土高原旱地小麦基于菌根群落结构优化下的氮素高效利用的可持续高产高效栽培技术体系提供依据。