基于打印有机薄膜晶体管的电化学阻抗免疫传感器研究

Immunosensor is a highly selective and sensitive sensing technology, which has been widely used in clinic diagnostics. However, conventional immunosensors employ bulky optical detection system and complex reagents preparation procedures. The portability of the sensing system has been become the bottleneck which stops immunosensors to be used in other areas. In order to address this issue, this project will develop an inkjet printed OTFT-based electrochemical impedance immunosensor. This will reduce the size of immunosensors from both the detection methodologies and hardware realisations aspects. It will prompt immunosensors' applications in point-of-care testing, food quality and environmental analysis. Electrochemical impedance immunosensing is using the impedance changes on the electrode-electrolyte interfaces to detect the protein-protein binding reactions. This label-free technology enables a minimized detection system setup with simple reagents. Meanwhile, low-cost inkjet printed OTFT technology has been highly developed in recent years. It has been already able to be used as the fundamental building blocks to realize the impedance sensing functions. This project will focus on printed thin-film device optimization, impedance sensing circuit design and immunosensing functions developments. It will provide a new era for the portable, low-cost immunosensing.

免疫传感器以其检测特异性强和敏感度高等特点，已经在临床医学诊断中得到了广泛使用。但受制于现有光学传感器系统复杂度和操作繁琐等因素，很难在需求便捷、快速的日常检测领域中实现具体应用。针对免疫传感器便携性这一问题，本项目提出了一种发展基于打印电路的电化学阻抗免疫传感器的新思路。试图同时在传感器方法学和传感器硬件上提高免疫检测系统的便携程度，从而将其进一步推广到在场疾病检测、食品安全和环境检测等领域。电化学阻抗免疫测试方法利用生物样本与电极界面的电学性质变化，无需利用任何标记物便可以直接对免疫反应进行识别，测试系统相对结构简单。同时，快速打印有机薄膜晶体管技术近年来高速发展，其技术成熟度已经可以满足电化学阻抗测试系统对于硬件的需求。本项目将从打印器件结构优化、阻抗测试电路设计、外围匹配电路设计和免疫功能验证等方面展开研究，为便携式、快速免疫检测技术提供一条解决途径。

本项目提出了一种发展新型薄膜电极器件的电化学阻抗免疫传感器的新思路。主要依托团队前期在薄膜半导体器件领域的研发基础，以先进薄膜半导体器件为核心功能模块，进行信号放大电路的设计及集成，并将其应用于电化学阻抗免疫传感器芯片中。本项目以喷墨打印有机薄膜晶体管为核心器件，进行了阻抗信号放大器的设计及功能表征、系统集成，证明了基于薄膜电子器件的放大器在电化学免疫传感器信号检测端应用的可能性。同时，在电化学传感器的核心--金属电极制备上，在本项目的支撑下，团队开发了一种基于激光直写的快速电极制备的技术手段，并实现了对于免疫球蛋白的高灵敏检测（检测限低至10 ng/ml）。此外，我们同时探索了阵列式阻抗传感器在其他应用中的前景，证明了其可以作为一种有效的手段对数字微流控芯片中的微滴样本进行识别；同时尝试了阵列式电化学阻抗芯片在除免疫外的不同生物检测领域（分子检测、细胞检测等）的应用探索；并最终以综述的形式，对此领域的工作进行了总结及展望，也为本团队后续的科研探索指明了方向。

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