泥鳅肠气呼吸功能的发生与发育及其分子调控机制

All the time, animal physiologist and animal development biologist has been attracted by the intestinal air-breathing phenomenon in Misgurnus anguillicaudatus. In order to understand the occurrence, development and its molecular regulatory mechanisms of intestinal air-breathing function of this fish, we will first use histological methods, electron microscopy techniques for direct observation of variations of intestine tissue structure during the period of intestinal development. Meanwhile, some important respiratory physiology parameters will be determined. The change of hindgut's function will be investigated by the fluorescence in situ hybridization method. In consequence, the occurrence and development process of intestinal air-breathing function in Misgurnus anguillicaudatus will be well known. A comparative study of gene expression profiles between the hindgut tissue of non-respiratory function and the hindgut tissue which just has respiratory function will be conducted. And then the key genes for regulating the intestine respiratory function will be screened out. We will clone the key genes. Gene expression and regulation analysis will be performed as well. At last, the molecular regulatory mechanisms in the occurrence and development of intestinal air-breathing function will be explored and the gene regulatory networks will be established. The results of this project will fill the research vacancy in the development study of fish accessory respiratory organs, and promote the research of vertebrate accessory respiratory organs. At the same time, they also benefit the prevention of 'flatulence disease', artificial high-density culture and artificial transportation of Misgurnus anguillicaudatus.

泥鳅肠气呼吸现象一直以来，都广受动物生理学家和发育学家的关注。为了探索泥鳅肠气呼吸功能发生与发育及其分子调控机制，本项目拟通过对泥鳅肠胚后发育的形态与组织学观察，同时辅以泥鳅胚后发育过程中的呼吸生理和肠道（指后肠）功能转变研究，了解泥鳅肠气呼吸功能的发生与发育过程。利用生物信息学分析方法比较研究肠气呼吸功能发生前的后肠组织与肠气呼吸功能发生后的后肠组织之间的基因表达谱，筛选出调控泥鳅肠气呼吸的关键基因，克隆关键基因并进行基因表达和调控分析，最后探讨泥鳅肠气呼吸功能发生发育的分子调控机制，建立基因调控网络。本项目研究结果将填补鱼类辅助呼吸器官发育学研究方向的空缺，推进对脊椎动物辅助呼吸器官的研究。同时，还能为泥鳅'胀气病'的防治、人工高密度养殖、以及人工运输等方面的研究提供理论基础。